Monkeypox DNA Polymerase: Difference between revisions
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'''Supporting biochemistry:''' Primer-extension assays show F8 alone is distributive (incorporates <14 nt), while addition/assembly with A22–E4 yields full-length extension (60 nt template) in a concentration-dependent manner; alanine scanning of E4 residues (e.g., W36, R39, N165) confirms residues critical for processivity. | '''Supporting biochemistry:''' Primer-extension assays show F8 alone is distributive (incorporates <14 nt), while addition/assembly with A22–E4 yields full-length extension (60 nt template) in a concentration-dependent manner; alanine scanning of E4 residues (e.g., W36, R39, N165) confirms residues critical for processivity. | ||
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The following figure shows two binding modes of processivity factors with polymerases. The processivity factors bound with template in poxvirus function as a “forward sliding clamp” (i) or dsDNA products in eukaryotes as a “backward sliding clamp” (ii). | |||
[[Image:MPXV.png|700px]] | [[Image:MPXV.png|700px]] | ||
