Halo Tag: Difference between revisions

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the dye back to its non-fluorescent state.
the dye back to its non-fluorescent state.


[[Image:Principle of HaloTag.jpg | thumb | upright | right | alt= | Fig.1. General principle of photoswitchable HaloTag (psHaloTag) and open-closed equilibrium of JF635-HaloTag ligand (HTL). L1 and L2 denote linkers. ]]
[[Image:Principle of HaloTag.jpg | thumb | upright= 1.5 |none | alt= | Fig.1. General principle of photoswitchable HaloTag (psHaloTag) and open-closed equilibrium of JF635-HaloTag ligand (HTL). L1 and L2 denote linkers. ]]


The psHaloTag variants show a strong, reversible, deep-red fluorescence turn-on when activated by light. This provides a well-controlled tool for improved imaging.
The psHaloTag variants show a strong, reversible, deep-red fluorescence turn-on when activated by light. This provides a well-controlled tool for improved imaging.
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