Halo Tag: Difference between revisions
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*Protein Scaffold: An engineered HaloTag protein with the light-sensing sAsLOV2 domain. | *Protein Scaffold: An engineered HaloTag protein with the light-sensing sAsLOV2 domain. | ||
*Ligand: Binds to a fluorogenic rhodamine dye (JF635-HTL). | *Ligand: Binds to a <scene name='10/1096855/Jf/1'>fluorogenic rhodamine dye (JF635-HTL)</scene>. | ||
:Light ON (450 nm): Light activates the sAsLOV2 domain using Flavin Mononucleotide (FMN) | :Light ON (450 nm): Light activates the sAsLOV2 domain using <scene name='10/1096855/Fmn/1'>Flavin Mononucleotide (FMN)</scene> | ||
cofactor, causing a conformational change (Jα helix unfolding). | cofactor, causing a conformational change (Jα helix unfolding). | ||
:Fluorescence ON: This change forces the bound dye into its fluorescent state. | :Fluorescence ON: This change forces the bound dye into its fluorescent state. | ||
