9nvs: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 9nvs is ~~ON HOLD until Paper~~ Publication

==Structure of a pentameric Nanchung in complex with Afidopyropen==

== Structural highlights ==

<table><tr><td colspan='2'>[[9nvs]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Halyomorpha_halys Halyomorpha halys]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9NVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9NVS FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.28Å</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1B32:[(1~{S},2~{S},5~{S},6~{R},7~{S},9~{S},10~{S},18~{R})-5-cyclopropylcarbonyloxy-2,6,10-trimethyl-9,18-bis(oxidanyl)-16-oxidanylidene-14-pyridin-3-yl-11,15-dioxatetracyclo[8.8.0.0^{2,7}.0^{12,17}]octadeca-12(17),13-dien-6-yl]methyl+cyclopropanecarboxylate'>A1B32</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9nvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9nvs OCA], [https://pdbe.org/9nvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9nvs RCSB], [https://www.ebi.ac.uk/pdbsum/9nvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9nvs ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Insecticides are vital to combating world food shortages and transmission of vector-borne human diseases. Increasing insecticide resistance necessitates discovery of novel compounds against underutilized targets. Nanchung (Nan) and Inactive (Iav), the transient receptor potential vanilloid-type (TRPV) channels in insects, likely form a heteromeric channel (Nan-Iav) and are localized in mechanosensory chordotonal organs which confer gravitaxis, hearing and proprioception. Several insecticides, such as afidopyropen (AP), target Nan-Iav through unknown mechanisms. Effective against piercing-sucking (hemipteran) insects, AP disrupts chordotonal functions preventing feeding. AP can bind to Nan alone, but only Nan-Iav exhibits channel activity with agonists including endogenous nicotinamide (NAM). Despite its importance as an insecticide target, much is unknown about Nan-Iav, such as channel assembly, modulator binding sites, and Ca(2+)-dependent regulation, hampering further insecticide development. Here we present the cryo-electron microscopy structures of hemipteran Nan-Iav with calmodulin bound in the apo state and with AP and NAM bound to cytosolic ankyrin repeat domain (ARD) interfaces. Unexpectedly, we found that Nan alone can form a pentamer, stabilized through AP-mediated ARD interactions. Our study provides molecular insights into insecticide and agonist interactions with Nan-Iav, highlighting the importance of the ARD on channel function and assembly, while also probing regulation by Ca(2+).

~~Authors~~:

Visualizing insecticide control of insect TRP channel function and assembly.,Fedor JG, Kandasamy R, Park CG, Suo Y, Weisel M, Rankl NB, Nesterov A, Lee SY Nat Commun. 2025 Dec 14;17(1):595. doi: 10.1038/s41467-025-67287-2. PMID:41392056<ref>PMID:41392056</ref>

~~Description~~:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Halyomorpha halys]]

[[Category: Large Structures]]

[[Category: Fedor JG]]

[[Category: Lee S-Y]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9nvs is ON HOLD  until Paper Publication
+==Structure of a pentameric Nanchung in complex with Afidopyropen==
+<StructureSection load='9nvs' size='340' side='right'caption='[[9nvs]], [[Resolution|resolution]] 3.28&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9nvs]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Halyomorpha_halys Halyomorpha halys]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9NVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9NVS FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.28&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1B32:[(1~{S},2~{S},5~{S},6~{R},7~{S},9~{S},10~{S},18~{R})-5-cyclopropylcarbonyloxy-2,6,10-trimethyl-9,18-bis(oxidanyl)-16-oxidanylidene-14-pyridin-3-yl-11,15-dioxatetracyclo[8.8.0.0^{2,7}.0^{12,17}]octadeca-12(17),13-dien-6-yl]methyl+cyclopropanecarboxylate'>A1B32</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9nvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9nvs OCA], [https://pdbe.org/9nvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9nvs RCSB], [https://www.ebi.ac.uk/pdbsum/9nvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9nvs ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Insecticides are vital to combating world food shortages and transmission of vector-borne human diseases. Increasing insecticide resistance necessitates discovery of novel compounds against underutilized targets. Nanchung (Nan) and Inactive (Iav), the transient receptor potential vanilloid-type (TRPV) channels in insects, likely form a heteromeric channel (Nan-Iav) and are localized in mechanosensory chordotonal organs which confer gravitaxis, hearing and proprioception. Several insecticides, such as afidopyropen (AP), target Nan-Iav through unknown mechanisms. Effective against piercing-sucking (hemipteran) insects, AP disrupts chordotonal functions preventing feeding. AP can bind to Nan alone, but only Nan-Iav exhibits channel activity with agonists including endogenous nicotinamide (NAM). Despite its importance as an insecticide target, much is unknown about Nan-Iav, such as channel assembly, modulator binding sites, and Ca(2+)-dependent regulation, hampering further insecticide development. Here we present the cryo-electron microscopy structures of hemipteran Nan-Iav with calmodulin bound in the apo state and with AP and NAM bound to cytosolic ankyrin repeat domain (ARD) interfaces. Unexpectedly, we found that Nan alone can form a pentamer, stabilized through AP-mediated ARD interactions. Our study provides molecular insights into insecticide and agonist interactions with Nan-Iav, highlighting the importance of the ARD on channel function and assembly, while also probing regulation by Ca(2+).
-Authors:
+Visualizing insecticide control of insect TRP channel function and assembly.,Fedor JG, Kandasamy R, Park CG, Suo Y, Weisel M, Rankl NB, Nesterov A, Lee SY Nat Commun. 2025 Dec 14;17(1):595. doi: 10.1038/s41467-025-67287-2. PMID:41392056<ref>PMID:41392056</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 9nvs" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Halyomorpha halys]]
+[[Category: Large Structures]]
+[[Category: Fedor JG]]
+[[Category: Lee S-Y]]