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'''Unreleased structure'''


The entry 9tax is ON HOLD  until Paper Publication
==Structure of the human inner kinetochore CCAN bound to a mono-CENP-A nucleosome==
<StructureSection load='9tax' size='340' side='right'caption='[[9tax]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9tax]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9TAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9TAX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9tax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9tax OCA], [https://pdbe.org/9tax PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9tax RCSB], [https://www.ebi.ac.uk/pdbsum/9tax PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9tax ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Human kinetochores assemble onto centromeric DNA comprising repetitive arrays of the 171 bp alpha-satellite sequence. To understand the higher-order architecture of the inner kinetochore assembled onto alpha-satellite arrays, we show cryo-EM structures of CCAN with free DNA, and alpha-satellite repeat monomers and dimers with CENP-A nucleosomes. CCAN bound to free DNA and a monomeric CENP-A nucleosome engages 70 bp of DNA comprising 30 bp of an upstream alpha-satellite repeat. This upstream DNA interacts with the histone-fold domain subunits of the CENP-TWSX module in a manner resembling how nucleosomes wrap DNA gyres. A complex of CCAN assembled onto a dimeric alpha-satellite repeat with two CENP-A nucleosomes shows that CCAN can only be accommodated on the linker DNA by unwrapping DNA from both the CENP-TWSX module and the upstream nucleosome. We discuss the implications of these results for models of CCAN assembly on arrays of alpha-satellite chromatin containing CENP-A nucleosomes.


Authors: Yu, C., Barford, D.
Models for the architecture of the human inner kinetochore on centromeric alpha-satellite CENP-A nucleosome arrays.,Yu C, Muir KW, Yang J, Zhang Z, McLaughlin SH, Barford D Nat Commun. 2026 May 12. doi: 10.1038/s41467-026-72856-0. PMID:42120391<ref>PMID:42120391</ref>


Description: Structure of the human inner kinetochore CCAN bound to a mono-CENP-A nucleosome
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Barford, D]]
<div class="pdbe-citations 9tax" style="background-color:#fffaf0;"></div>
[[Category: Yu, C]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Barford D]]
[[Category: Yu C]]
Retrieved from "https://proteopedia.org/9tax"