9z7v: Difference between revisions

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'''Unreleased structure'''


The entry 9z7v is ON HOLD  until Paper Publication
==Cryo-EM Structure of the Type III-Bv CRISPR Complex from Dissulfurispira thermophila bound to target RNA with complementary PFS==
<StructureSection load='9z7v' size='340' side='right'caption='[[9z7v]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9z7v]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Dissulfurispira_thermophila Dissulfurispira thermophila] and [https://en.wikipedia.org/wiki/Escherichia_phage_MS2 Escherichia phage MS2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9Z7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9Z7V FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9z7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9z7v OCA], [https://pdbe.org/9z7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9z7v RCSB], [https://www.ebi.ac.uk/pdbsum/9z7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9z7v ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A7G1H3Q2_9BACT A0A7G1H3Q2_9BACT]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cas7-family proteins form the scaffolds of multi-subunit CRISPR RNA-guided surveillance complexes. To explore how Cas7 diversification expands CRISPR function, we identified Cas7 fusion proteins linked to diverse accessory domains, including a type III-B variant (III-Bv) in which a Cas7 homolog (Cmr1) is fused to the MntA antitoxin and encoded adjacent to a HEPN-family toxin. Structures reveal that the core Cas proteins assemble into a stable surveillance complex in the absence of crRNA, whereas incorporation of the Cmr1-MntA fusion is crRNA-dependent. Target RNA recognition triggers conformational changes that expose the Cas10 cyclase active site and promote cyclic oligoadenylate synthesis. Biochemical analyses show that the CRISPR-associated MntA is enzymatically active and AMPylates the associated HEPN protein. Together, these findings establish the structural basis for assembly of a type III-Bv surveillance complex containing an enzymatically active toxin-antitoxin module.


Authors: Burman, N., Pandey, S., Wiedenheft, B.
Identification and structure determination of a type III-Bv CRISPR complex that post-translationally modifies an associated toxin.,Pandey S, Burman N, Henriques WS, Wiegand T, Zahl T, Nyquist H, Spreeuw T, Buyukyoruk M, Wiedenheft B Structure. 2026 Jul 1:S0969-2126(26)00183-8. doi: 10.1016/j.str.2026.06.002. PMID:42385699<ref>PMID:42385699</ref>


Description: Cryo-EM Structure of the Type III-Bv CRISPR Complex from Dissulfurispira thermophila bound to target RNA with complementary PFS
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Wiedenheft, B]]
<div class="pdbe-citations 9z7v" style="background-color:#fffaf0;"></div>
[[Category: Pandey, S]]
== References ==
[[Category: Burman, N]]
<references/>
__TOC__
</StructureSection>
[[Category: Dissulfurispira thermophila]]
[[Category: Escherichia phage MS2]]
[[Category: Large Structures]]
[[Category: Burman N]]
[[Category: Pandey S]]
[[Category: Wiedenheft B]]
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