9zbu: Difference between revisions

Latest revision as of 05:16, 27 May 2026

Visualization of PriA/PriB/DnaT complexes reveals mechanisms governing structure-specific assembly of the DNA replication restart primosome

Structural highlights

9zbu is a 13 chain structure with sequence from Escherichia coli K-12 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.6Å
Ligands:	ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PRIB_ECOLI Binds single-stranded DNA at the primosome assembly site (PAS). During primosome assembly it facilitates the complex formation between PriA and DnaT.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Replication restart pathways reinitiate DNA replication processes following their premature termination. In Escherichia coli, this essential process begins with regulated assembly of the preprimosome complex, comprising the PriA, PriB, and DnaT proteins, onto an abandoned replication fork. Here, we present two distinct preprimosome structures. One represents an intermediate stage in preprimosome assembly with a single DnaT C-terminal domain (DnaT(CTD)) bound to PriA/PriB/DNA. The second captures the mature preprimosome, in which filamentation of multiple DnaT(CTD) molecules catalyzes the handoff of the single-stranded lagging-strand DNA from PriB to DnaT. The DnaT N-terminal domain forms a separate, independent oligomer in the mature structure. Taken together, our results detail the molecular mechanisms underlying replication restart initiation and regulation and suggest mechanistic similarities between DnaT and the canonical initiator protein DnaA.

Visualization of the complete preprimosome reveals the structural mechanisms governing DNA replication restart.,Ducos PL, Duckworth AT, Satyshur KA, Keck JL, Grant T Nat Commun. 2026 May 16. doi: 10.1038/s41467-026-73239-1. PMID:42140935^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zavitz KH, DiGate RJ, Marians KJ. The priB and priC replication proteins of Escherichia coli. Genes, DNA sequence, overexpression, and purification. J Biol Chem. 1991 Jul 25;266(21):13988-95. PMID:1856227
↑ Allen GC Jr, Kornberg A. The priB gene encoding the primosomal replication n protein of Escherichia coli. J Biol Chem. 1991 Jun 25;266(18):11610-3. PMID:1646811
↑ Allen GC Jr, Kornberg A. Assembly of the primosome of DNA replication in Escherichia coli. J Biol Chem. 1993 Sep 15;268(26):19204-9. PMID:8366072
↑ Ducos PL, Duckworth AT, Satyshur KA, Keck JL, Grant T. Visualization of the complete preprimosome reveals the structural mechanisms governing DNA replication restart. Nat Commun. 2026 May 16;17(1):6489. PMID:42140935 doi:10.1038/s41467-026-73239-1

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OCA

[1] Zavitz KH, DiGate RJ, Marians KJ. The priB and priC replication proteins of Escherichia coli. Genes, DNA sequence, overexpression, and purification. J Biol Chem. 1991 Jul 25;266(21):13988-95. PMID:1856227

[2] Allen GC Jr, Kornberg A. The priB gene encoding the primosomal replication n protein of Escherichia coli. J Biol Chem. 1991 Jun 25;266(18):11610-3. PMID:1646811

[3] Allen GC Jr, Kornberg A. Assembly of the primosome of DNA replication in Escherichia coli. J Biol Chem. 1993 Sep 15;268(26):19204-9. PMID:8366072

[4] Ducos PL, Duckworth AT, Satyshur KA, Keck JL, Grant T. Visualization of the complete preprimosome reveals the structural mechanisms governing DNA replication restart. Nat Commun. 2026 May 16;17(1):6489. PMID:42140935 doi:10.1038/s41467-026-73239-1

[1]

[2]

[3]

[4]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 9zbu is ON HOLD  until Paper Publication
+==Visualization of PriA/PriB/DnaT complexes reveals mechanisms governing structure-specific assembly of the DNA replication restart primosome==
+<StructureSection load='9zbu' size='340' side='right'caption='[[9zbu]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[9zbu]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9ZBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9ZBU FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9zbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9zbu OCA], [https://pdbe.org/9zbu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9zbu RCSB], [https://www.ebi.ac.uk/pdbsum/9zbu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9zbu ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PRIB_ECOLI PRIB_ECOLI] Binds single-stranded DNA at the primosome assembly site (PAS). During primosome assembly it facilitates the complex formation between PriA and DnaT.<ref>PMID:1856227</ref> <ref>PMID:1646811</ref> <ref>PMID:8366072</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Replication restart pathways reinitiate DNA replication processes following their premature termination. In Escherichia coli, this essential process begins with regulated assembly of the preprimosome complex, comprising the PriA, PriB, and DnaT proteins, onto an abandoned replication fork. Here, we present two distinct preprimosome structures. One represents an intermediate stage in preprimosome assembly with a single DnaT C-terminal domain (DnaT(CTD)) bound to PriA/PriB/DNA. The second captures the mature preprimosome, in which filamentation of multiple DnaT(CTD) molecules catalyzes the handoff of the single-stranded lagging-strand DNA from PriB to DnaT. The DnaT N-terminal domain forms a separate, independent oligomer in the mature structure. Taken together, our results detail the molecular mechanisms underlying replication restart initiation and regulation and suggest mechanistic similarities between DnaT and the canonical initiator protein DnaA.
-Authors: Duckworth, A.T., Keck, J.L., Grant, T.
+Visualization of the complete preprimosome reveals the structural mechanisms governing DNA replication restart.,Ducos PL, Duckworth AT, Satyshur KA, Keck JL, Grant T Nat Commun. 2026 May 16. doi: 10.1038/s41467-026-73239-1. PMID:42140935<ref>PMID:42140935</ref>
-Description: Visualization of PriA/PriB/DnaT complexes reveals mechanisms governing structure-specific assembly of the DNA replication restart primosome
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Grant, T]]
+<div class="pdbe-citations 9zbu" style="background-color:#fffaf0;"></div>
-[[Category: Keck, J.L]]
+== References ==
-[[Category: Duckworth, A.T]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli K-12]]
+[[Category: Large Structures]]
+[[Category: Synthetic construct]]
+[[Category: Duckworth AT]]
+[[Category: Grant T]]
+[[Category: Keck JL]]

9zbu: Difference between revisions

Latest revision as of 05:16, 27 May 2026

Visualization of PriA/PriB/DnaT complexes reveals mechanisms governing structure-specific assembly of the DNA replication restart primosome

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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