22xm: Difference between revisions
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==MexBYB-Ka symmetry-like== | |||
<StructureSection load='22xm' size='340' side='right'caption='[[22xm]], [[Resolution|resolution]] 3.55Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[22xm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=22XM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=22XM FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.55Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=22xm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=22xm OCA], [https://pdbe.org/22xm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=22xm RCSB], [https://www.ebi.ac.uk/pdbsum/22xm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=22xm ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
RND-type multidrug-efflux pumps are major contributors to multidrug resistance in Gram-negative bacteria, with MexY from Pseudomonas aeruginosa playing a central role in aminoglycoside resistance. Unlike other RND transporters, MexY exhibits unusually large open clefts in the binding and extrusion states. To determine whether this feature is intrinsic to its drug-recognition porter domain, we created a chimeric protein, MexBYB, by replacing the funnel-like and transmembrane domains of MexY with those of the homologous transporter MexB, and determined its structures by cryoEM under apo and kanamycin-supplemented conditions. Under both conditions, MexBYB was reported to adopt symmetric-like and asymmetric conformations. Structural comparisons reveal that the unusually large open clefts are retained in MexBYB, indicating that this feature is intrinsic to the MexY porter domain. | |||
Cryo-EM structures of a MexB-MexY chimeric efflux pump reveal that large open clefts are intrinsic to the MexY porter domain.,Wang J, Tsutsumi K, Hirose M, Nakashima R, Kato T, Nishino K, Nakagawa A, Yamashita E Acta Crystallogr F Struct Biol Commun. 2026 Mar 1;82(Pt 3):83-93. doi: , 10.1107/S2053230X26001202. Epub 2026 Feb 26. PMID:41744473<ref>PMID:41744473</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 22xm" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas aeruginosa]] | |||
[[Category: Nakagawa A]] | |||
[[Category: Wang J]] | |||
[[Category: Yamashita E]] | |||
