1xc4: Difference between revisions

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[[Image:1xc4.jpg|left|200px]]
{{Seed}}
[[Image:1xc4.png|left|200px]]


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{{STRUCTURE_1xc4|  PDB=1xc4  |  SCENE=  }}  
{{STRUCTURE_1xc4|  PDB=1xc4  |  SCENE=  }}  


'''Crystal structure of wild-type tryptophan synthase alpha-subunits from Escherichia coli'''
===Crystal structure of wild-type tryptophan synthase alpha-subunits from Escherichia coli===




==Overview==
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The alpha-subunit of tryptophan synthase (alphaTS) catalyzes the cleavage of indole-3-glycerol phosphate to glyceraldehyde-3-phosphate and indole, which is used to yield the amino acid tryptophan in tryptophan biosynthesis. Here, we report the first crystal structures of wild-type and double-mutant P28L/Y173F alpha-subunit of tryptophan synthase from Escherichia coli at 2.8 and 1.8A resolution, respectively. The structure of wild-type alphaTS from E. coli was similar to that of the alpha(2)beta(2) complex structure from Salmonella typhimurium. As compared with both structures, the conformational changes are mostly in the interface of alpha- and beta-subunits, and the substrate binding region. Two sulfate ions and two glycerol molecules per asymmetric unit bind with the residues in the active sites of the wild-type structure. Contrarily, double-mutant P28L/Y173F structure is highly closed at the window for the substrate binding by the conformational changes. The P28L substitution induces the exposure of hydrophobic amino acids and decreases the secondary structure that causes the aggregation. The Y173F suppresses to transfer a signal from the alpha-subunit core to the alpha-subunit surface involved in interactions with the beta-subunit and increases structural stability.
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==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structures of wild-type and P28L/Y173F tryptophan synthase alpha-subunits from Escherichia coli., Jeong MS, Jeong JK, Lim WK, Jang SB, Biochem Biophys Res Commun. 2004 Oct 29;323(4):1257-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15451433 15451433]
Structures of wild-type and P28L/Y173F tryptophan synthase alpha-subunits from Escherichia coli., Jeong MS, Jeong JK, Lim WK, Jang SB, Biochem Biophys Res Commun. 2004 Oct 29;323(4):1257-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15451433 15451433]
Three-dimensional structure of the tryptophan synthase alpha 2 beta 2 multienzyme complex from Salmonella typhimurium., Hyde CC, Ahmed SA, Padlan EA, Miles EW, Davies DR, J Biol Chem. 1988 Nov 25;263(33):17857-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3053720 3053720]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Tryptophan synthase]]
[[Category: Tryptophan synthase]]
[[Category: Wild-type]]
[[Category: Wild-type]]
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