|
|
| Line 1: |
Line 1: |
| [[Image:1xck.gif|left|200px]] | | {{Seed}} |
| | [[Image:1xck.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1xck| PDB=1xck | SCENE= }} | | {{STRUCTURE_1xck| PDB=1xck | SCENE= }} |
|
| |
|
| '''Crystal structure of apo GroEL'''
| | ===Crystal structure of apo GroEL=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| The 2.9A resolution crystal structure of apo wild-type GroEL was determined for the first time and represents the reference structure, facilitating the study of structural and functional differences observed in GroEL variants. Until now the crystal structure of the mutant Arg13Gly, Ala126Val GroEL was used for this purpose. We show that, due to the mutations as well as to the presence of a crystallographic symmetry, the ring-ring interface was inaccurately described. Analysis of the present structure allowed the definition of structural elements at this interface, essential for understanding the inter-ring allosteric signal transmission. We also show unambiguously that there is no ATP-induced 102 degrees rotation of the apical domain helix I around its helical axis, as previously assumed in the crystal structure of the (GroEL-KMgATP)(14) complex, and analyze the apical domain movements. These results enabled us to compare our structure with other GroEL crystal structures already published, allowing us to suggest a new route through which the allosteric signal for negative cooperativity propagates within the molecule. The proposed mechanism, supported by known mutagenesis data, underlines the importance of the switching of salt bridges. | | The line below this paragraph, {{ABSTRACT_PUBMED_16288915}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16288915 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_16288915}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 28: |
Line 32: |
| [[Category: Manakova, E.]] | | [[Category: Manakova, E.]] |
| [[Category: Chaperonin]] | | [[Category: Chaperonin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:51:25 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:24:05 2008'' |
