22jw: Difference between revisions
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==Perinereis linea erythrocruorin== | |||
<StructureSection load='22jw' size='340' side='right'caption='[[22jw]], [[Resolution|resolution]] 2.84Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[22jw]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Perinereis_linea Perinereis linea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=22JW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=22JW FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.84Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=22jw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=22jw OCA], [https://pdbe.org/22jw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=22jw RCSB], [https://www.ebi.ac.uk/pdbsum/22jw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=22jw ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Many invertebrates lack erythrocytes and instead rely on extracellular hemoglobin assemblies, termed erythrocruorins, for oxygen transport. Here we report a 2.84 A cryo-electron microscopy (cryo-EM) structure of Perinereis linea erythrocruorin (PlEc). PlEc is a approximately 3.3 MDa megacomplex composed of 180 polypeptide chains organized into 12 protomers, forming a hexagonal bilayer with D6 symmetry. Each protomer consists of 12 globin subunits and three linker subunits, adopting a mushroom-like architecture. The cap of the mushroom is formed by a globin dodecamer associated with a heterotrimeric linker head, and the stem consists of a triple-stranded coiled coil derived from the N-terminal helices of three linker subunits. Biochemical assays show that PlEc has thermal stability and auto-oxidation rate comparable to those of other erythrocruorins, but displays relatively lower oxygen-binding affinity. These findings provide mechanistic insights into the quaternary assembly of invertebrate erythrocruorins and lay the groundwork for the potential biomedical applications. | |||
Structure of Perinereis linea erythrocruorin reveals a compact extracellular globin megacomplex.,Deng JX, Cheng WB, Xu K, Hou P, Chen Y, Jiang YL, Zhou CZ Structure. 2026 Aug 6;34(8):1123-1133.e3. doi: 10.1016/j.str.2026.06.004. Epub , 2026 Jul 3. PMID:42398506<ref>PMID:42398506</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 22jw" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Perinereis linea]] | |||
[[Category: Deng JX]] | |||
[[Category: Jiang YL]] | |||
[[Category: Zhou CZ]] | |||
