9wt1: Difference between revisions

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'''Unreleased structure'''


The entry 9wt1 is ON HOLD  until Paper Publication
==CryoEM structure of cap module in the contracted AlgoCIS==
<StructureSection load='9wt1' size='340' side='right'caption='[[9wt1]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9wt1]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Algoriphagus_machipongonensis Algoriphagus machipongonensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WT1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wt1 OCA], [https://pdbe.org/9wt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wt1 RCSB], [https://www.ebi.ac.uk/pdbsum/9wt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wt1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A3HTC4_9BACT A3HTC4_9BACT]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Contractile injection systems (CISs) mediate cell-cell interactions and are widespread among bacteria and archaea. These phage tail-like macromolecular machines puncture their target by a tube that is propelled by a contractile sheath. The mechanism underlying CIS firing, which starts with target binding and ends with sheath contraction, remains unclear. Here, we investigate the extracellular CIS from Algoriphagus machipongonensis (AlgoCIS) by a multimodal cryo-electron microscopy approach and structure-guided engineering, which allowed us to arrest AlgoCIS in multiple intermediate states of firing. Together with the post-firing structure, our data suggest a stepwise firing mechanism involving all structural modules: signal propagation starts with the binding of the tail-fibers, followed by opening of the cage, an expansion of the baseplate iris, and resulting in sheath contraction and the release of cap adaptor. Our study will serve as a framework for understanding the firing mechanism of diverse CISs and will facilitate the engineering of CISs for biomedical applications.


Authors:  
Stepwise firing mechanism of an extracellular contractile injection system.,Xu J, Ericson CF, Toenshoff ER, Pilhofer M Nat Commun. 2026 Apr 24. doi: 10.1038/s41467-026-72240-y. PMID:42031783<ref>PMID:42031783</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 9wt1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Algoriphagus machipongonensis]]
[[Category: Large Structures]]
[[Category: Ericson CF]]
[[Category: Pilhofer M]]
[[Category: Toenshoff ER]]
[[Category: Xu J]]
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