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[[Image:1xty.gif|left|200px]]
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[[Image:1xty.png|left|200px]]


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{{STRUCTURE_1xty|  PDB=1xty  |  SCENE=  }}  
{{STRUCTURE_1xty|  PDB=1xty  |  SCENE=  }}  


'''Crystal structure of Sulfolobus solfataricus peptidyl-tRNA hydrolase'''
===Crystal structure of Sulfolobus solfataricus peptidyl-tRNA hydrolase===




==Overview==
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The 3-D structure of the peptidyl-tRNA hydrolase from the archaea Sulfolobus solfataricus has been solved at 1.8 A resolution. Homologues of this enzyme are found in archaea and eucarya. Bacteria display a different type of peptidyl-tRNA hydrolase that is also encountered in eucarya. In solution, the S. solfataricus hydrolase behaves as a dimer. In agreement, the crystalline structure of this enzyme indicates the formation of a dimer. Each protomer is made of a mixed five-stranded beta-sheet surrounded by two groups of two alpha-helices. The dimer interface is mainly formed by van der Waals interactions between hydrophobic residues belonging to the two N-terminal alpha1 helices contributed by two protomers. Site-directed mutagenesis experiments were designed for probing the basis of specificity of the archaeal hydrolase. Among the strictly conserved residues within the archaeal/eucaryal peptidyl-tRNA hydrolase family, three residues, K18, D86, and T90, appear of utmost importance for activity. They are located in the N-part of alpha1 and in the beta3-beta4 loop. K18 and D86, which form a salt bridge, might play a role in the catalysis thanks to their acid and basic functions, whereas the OH group of T90 could act as a nucleophile. These observations clearly distinguish the active site of the archaeal/eucaryal hydrolases from that of the bacterial/eucaryal ones, where a histidine is believed to serve as the catalytic base.
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{{ABSTRACT_PUBMED_15766258}}


==About this Structure==
==About this Structure==
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[[Category: Schmitt, E.]]
[[Category: Schmitt, E.]]
[[Category: Mixed beta sheet]]
[[Category: Mixed beta sheet]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 15:30:18 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:40:26 2008''

Revision as of 13:40, 29 July 2008

File:1xty.png

Template:STRUCTURE 1xty

Crystal structure of Sulfolobus solfataricus peptidyl-tRNA hydrolase

Template:ABSTRACT PUBMED 15766258

About this Structure

1XTY is a Single protein structure of sequence from Pyrococcus abyssi. Full crystallographic information is available from OCA.

Reference

Crystal structure at 1.8 A resolution and identification of active site residues of Sulfolobus solfataricus peptidyl-tRNA hydrolase., Fromant M, Schmitt E, Mechulam Y, Lazennec C, Plateau P, Blanquet S, Biochemistry. 2005 Mar 22;44(11):4294-301. PMID:15766258

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