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[[Image:1xvw.gif|left|200px]]
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[[Image:1xvw.png|left|200px]]


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{{STRUCTURE_1xvw|  PDB=1xvw  |  SCENE=  }}  
{{STRUCTURE_1xvw|  PDB=1xvw  |  SCENE=  }}  


'''Crystal Structure of AhpE from Mycobacterium tuberculosis, a 1-Cys peroxiredoxin'''
===Crystal Structure of AhpE from Mycobacterium tuberculosis, a 1-Cys peroxiredoxin===




==Overview==
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All living systems require protection against the damaging effects of reactive oxygen species. The genome of Mycobacterium tuberculosis, the cause of TB, encodes a number of peroxidases that are thought to be active against organic and inorganic peroxides, and are likely to play a key role in the ability of this organism to survive within the phagosomes of macrophages. The open reading frame Rv2238c in M.tuberculosis encodes a 153-residue protein AhpE, which is a peroxidase of the 1-Cys peroxiredoxin (Prx) family. The crystal structure of AhpE, determined at 1.87 A resolution (R(cryst)=0.179, R(free)=0.210), reveals a compact single-domain protein with a thioredoxin fold. AhpE forms both dimers and octamers; a tightly-associated dimer and a ring-like octamer, generated by crystallographic 4-fold symmetry. In this native structure, the active site Cys45 is in its oxidized, sulfenic acid (S-O-H) state. A second crystal form of AhpE, obtained after soaking in sodium bromide and refined at 1.90 A resolution (R(cryst)=0.242, R(free)=0.286), reveals the reduced structure. In this structure, a conformational change in an external loop, in two of the four molecules in the asymmetric unit, allows Arg116 to stabilise the Cys45 thiolate ion, and concomitantly closes a surface channel. This channel is identified as the likely binding site for a physiological reductant, and the conformational change is inferred to be important for the reaction cycle of AhpE.
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{{ABSTRACT_PUBMED_15701515}}


==About this Structure==
==About this Structure==
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[[Category: Tbsgc]]
[[Category: Tbsgc]]
[[Category: Thioredoxin fold]]
[[Category: Thioredoxin fold]]
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Revision as of 13:22, 27 July 2008

File:1xvw.png

Template:STRUCTURE 1xvw

Crystal Structure of AhpE from Mycobacterium tuberculosis, a 1-Cys peroxiredoxin

Template:ABSTRACT PUBMED 15701515

About this Structure

1XVW is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Crystal Structure of AhpE from Mycobacterium tuberculosis, a 1-Cys peroxiredoxin., Li S, Peterson NA, Kim MY, Kim CY, Hung LW, Yu M, Lekin T, Segelke BW, Lott JS, Baker EN, J Mol Biol. 2005 Mar 4;346(4):1035-46. Epub 2005 Jan 25. PMID:15701515

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