29zh: Difference between revisions
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==Crystal structure of human NIF3L1 - half open hexamer== | |||
<StructureSection load='29zh' size='340' side='right'caption='[[29zh]], [[Resolution|resolution]] 3.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[29zh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=29ZH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=29ZH FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=29zh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=29zh OCA], [https://pdbe.org/29zh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=29zh RCSB], [https://www.ebi.ac.uk/pdbsum/29zh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=29zh ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/NIF3L_HUMAN NIF3L_HUMAN] May function as a transcriptional corepressor through its interaction with COPS2, negatively regulating the expression of genes involved in neuronal differentiation.[UniProtKB:Q9EQ80] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
NIF3L1 (also NIF3) is a highly conserved protein belonging to the DUF34 protein family with unknown molecular function, present in bacteria, archaea, and eukaryotes. Here, we present the first crystal structures of human NIF3, revealing a hexameric toroidal assembly with a central cavity gated by PII-like insertion domains. Each subunit contains a mononuclear zinc-binding site, marking a clear departure from the dinuclear metal centers of bacterial homologs and potential functional divergence of the scaffold. We further capture a half-capped state with asymmetric disorder of the insertion domains and local rearrangements near the metal site, suggesting a gating mechanism that regulates access to the internal chamber. These findings uncover an unexpected level of structural plasticity in human NIF3 and provide a framework for future studies investigating whether the different lid conformations observed in our structures have functional relevance in vivo. | |||
Crystal structure of human NIF3-like protein reveals dynamic hexameric assembly with a single divalent metal binding site.,Wator-Wilk E, Zak K, Wilk P, Kochanowski P, Maslanka A, Skalniak L, Grudnik P FEBS J. 2026 Sep 15. doi: 10.1111/febs.70724. PMID:42741873<ref>PMID:42741873</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 29zh" style="background-color:#fffaf0;"></div> | ||
[[Category: Wator-Wilk | == References == | ||
[[Category: | <references/> | ||
[[Category: Zak | __TOC__ | ||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Grudnik P]] | |||
[[Category: Wator-Wilk E]] | |||
[[Category: Wilk P]] | |||
[[Category: Zak KM]] | |||
Latest revision as of 16:18, 25 September 2026
Crystal structure of human NIF3L1 - half open hexamer
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