|
|
| Line 1: |
Line 1: |
| [[Image:1y29.gif|left|200px]] | | {{Seed}} |
| | [[Image:1y29.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1y29| PDB=1y29 | SCENE= }} | | {{STRUCTURE_1y29| PDB=1y29 | SCENE= }} |
|
| |
|
| '''Three dimensional solution structure of huwentoxin-x by 2D 1H-NMR'''
| | ===Three dimensional solution structure of huwentoxin-x by 2D 1H-NMR=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Huwentoxin-X (HWTX-X) is a novel peptide toxin, purified from the venom of the spider Ornithoctonus huwena. It comprises 28 amino acid residues including six cysteine residues as disulfide bridges linked in the pattern of I-IV, II-V, and III-VI. Its cDNA, determined by rapid amplification of 3' and 5' cDNA ends, encodes a 65-residue prepropeptide. HWTX-X shares low sequence homology with omega-conotoxins GVIA and MVIIA, two well known blockers of N-type Ca2+ channels. Nevertheless, whole cell studies indicate that it can block N-type Ca2+ channels in rat dorsal root ganglion cells (IC50 40 nm) and the blockage by HWTX-X is completely reversible. The rank order of specificity for N-type Ca2+ channels is GVIA approximately HWTX-X > MVIIA. In contrast to GVIA and MVIIA, HWTX-X had no detectable effect on the twitch response of rat vas deferens to low frequency electrical stimulation, indicating that HWTX-X has different selectivity for isoforms of N-type Ca2+ channels, compared with GVIA or MVIIA. A comparison of the structures of HWTX-X and GVIA reveals that they not only adopt a common structural motif (inhibitor cystine knot), but also have a similar functional motif, a binding surface formed by the critical residue Tyr, and several basic residues. However, the dissimilarities of their binding surfaces provide some insights into their different selectivities for isoforms of N-type Ca2+ channels.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16439354}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16439354 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_16439354}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| 1Y29 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y29 OCA]. | | 1Y29 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y29 OCA]. |
|
| |
|
| ==Reference== | | ==Reference== |
| Line 26: |
Line 30: |
| [[Category: Ick]] | | [[Category: Ick]] |
| [[Category: Knottin]] | | [[Category: Knottin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:47:48 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:29:57 2008'' |
