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| [[Image:1y3a.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1y3a| PDB=1y3a | SCENE= }} | | {{STRUCTURE_1y3a| PDB=1y3a | SCENE= }} |
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| '''Structure of G-Alpha-I1 bound to a GDP-selective peptide provides insight into guanine nucleotide exchange'''
| | ===Structure of G-Alpha-I1 bound to a GDP-selective peptide provides insight into guanine nucleotide exchange=== |
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| ==Overview==
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| Heterotrimeric G proteins are molecular switches that regulate numerous signaling pathways involved in cellular physiology. This characteristic is achieved by the adoption of two principal states: an inactive, GDP bound state and an active, GTP bound state. Under basal conditions, G proteins exist in the inactive, GDP bound state; thus, nucleotide exchange is crucial to the onset of signaling. Despite our understanding of G protein signaling pathways, the mechanism of nucleotide exchange remains elusive. We employed phage display technology to identify nucleotide state-dependent Galpha binding peptides. Herein, we report a GDP-selective Galpha binding peptide, KB-752, that enhances spontaneous nucleotide exchange of Galpha(i) subunits. Structural determination of the Galpha(i1)/peptide complex reveals unique changes in the Galpha switch regions predicted to enhance nucleotide exchange by creating a GDP dissociation route. Our results cast light onto a potential mechanism by which Galpha subunits adopt a conformation suitable for nucleotide exchange.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16004878}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16004878 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16004878}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Willard, F S.]] | | [[Category: Willard, F S.]] |
| [[Category: Protein-peptide complex]] | | [[Category: Protein-peptide complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:49:56 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:58:33 2008'' |
