1yf3: Difference between revisions

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[[Image:1yf3.~~gif~~|left|200px]]

[[Image:1yf3.png|left|200px]]

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~~'''~~T4Dam in Complex with AdoHcy and 13-mer Oligonucleotide Making Non- and Semi-specific (~1/4) Contact~~'''~~

===T4Dam in Complex with AdoHcy and 13-mer Oligonucleotide Making Non- and Semi-specific (~1/4) Contact===

~~==Overview==~~

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~~DNA methyltransferases methylate target bases within specific nucleotide sequences. Three structures are described for bacteriophage T4 DNA~~-~~adenine methyltransferase (T4Dam) in ternary complexes with partially and fully specific DNA and a methyl~~-~~donor analog. We also report~~ the ~~effects of substitutions in~~ the ~~related Escherichia coli DNA methyltransferase~~ (~~EcoDam~~), ~~altering residues corresponding to those involved in specific interaction with~~ the ~~canonical GATC target sequence in T4Dam~~. ~~We have identified two types of protein~~-DNA interactions: discriminatory contacts, which stabilize the transition state and accelerate methylation of the cognate site, and antidiscriminatory contacts, which do not significantly affect methylation of the cognate site but disfavor activity at noncognate sites. These structures illustrate the transition in enzyme-~~DNA interaction from nonspecific to specific interaction, suggesting that there is a temporal order for formation of specific contacts.~~

The line below this paragraph, {{ABSTRACT_PUBMED_15882618}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 15882618 is the PubMed ID number.

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==About this Structure==

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[[Category: Methyltransferase]]

[[Category: T4dam]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3~~ 16:~~14:36~~ 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:16:10 2008''

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-[[Image:1yf3.gif|left|200px]]
+{{Seed}}
+[[Image:1yf3.png|left|200px]]
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 {{STRUCTURE_1yf3|  PDB=1yf3  |  SCENE=  }}
-'''T4Dam in Complex with AdoHcy and 13-mer Oligonucleotide Making Non- and Semi-specific (~1/4) Contact'''
+===T4Dam in Complex with AdoHcy and 13-mer Oligonucleotide Making Non- and Semi-specific (~1/4) Contact===
-==Overview==
+<!--
-DNA methyltransferases methylate target bases within specific nucleotide sequences. Three structures are described for bacteriophage T4 DNA-adenine methyltransferase (T4Dam) in ternary complexes with partially and fully specific DNA and a methyl-donor analog. We also report the effects of substitutions in the related Escherichia coli DNA methyltransferase (EcoDam), altering residues corresponding to those involved in specific interaction with the canonical GATC target sequence in T4Dam. We have identified two types of protein-DNA interactions: discriminatory contacts, which stabilize the transition state and accelerate methylation of the cognate site, and antidiscriminatory contacts, which do not significantly affect methylation of the cognate site but disfavor activity at noncognate sites. These structures illustrate the transition in enzyme-DNA interaction from nonspecific to specific interaction, suggesting that there is a temporal order for formation of specific contacts.
+The line below this paragraph, {{ABSTRACT_PUBMED_15882618}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15882618 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15882618}}
 ==About this Structure==
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 [[Category: Methyltransferase]]
 [[Category: T4dam]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 16:14:36 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:16:10 2008''