1yl3: Difference between revisions

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[[Image:1yl3.gif|left|200px]]
{{Seed}}
[[Image:1yl3.png|left|200px]]


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{{STRUCTURE_1yl3|  PDB=1yl3  |  SCENE=  }}  
{{STRUCTURE_1yl3|  PDB=1yl3  |  SCENE=  }}  


'''Crystal structure of 70S ribosome with thrS operator and tRNAs. Large subunit. The coordinates for the small subunit are in the pdb entry 1YL4.'''
===Crystal structure of 70S ribosome with thrS operator and tRNAs. Large subunit. The coordinates for the small subunit are in the pdb entry 1YL4.===




==Overview==
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The ribosome of Thermus thermophilus was cocrystallized with initiator transfer RNA (tRNA) and a structured messenger RNA (mRNA) carrying a translational operator. The path of the mRNA was defined at 5.5 angstroms resolution by comparing it with either the crystal structure of the same ribosomal complex lacking mRNA or with an unstructured mRNA. A precise ribosomal environment positions the operator stem-loop structure perpendicular to the surface of the ribosome on the platform of the 30S subunit. The binding of the operator and of the initiator tRNA occurs on the ribosome with an unoccupied tRNA exit site, which is expected for an initiation complex. The positioning of the regulatory domain of the operator relative to the ribosome elucidates the molecular mechanism by which the bound repressor switches off translation. Our data suggest a general way in which mRNA control elements must be placed on the ribosome to perform their regulatory task.
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{{ABSTRACT_PUBMED_15802605}}


==About this Structure==
==About this Structure==
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[[Category: Ribosome]]
[[Category: Ribosome]]
[[Category: Translational operator]]
[[Category: Translational operator]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 16:27:22 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:58:53 2008''

Revision as of 13:58, 28 July 2008

File:1yl3.png

Template:STRUCTURE 1yl3

Crystal structure of 70S ribosome with thrS operator and tRNAs. Large subunit. The coordinates for the small subunit are in the pdb entry 1YL4.

Template:ABSTRACT PUBMED 15802605

About this Structure

1YL3 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Translational operator of mRNA on the ribosome: how repressor proteins exclude ribosome binding., Jenner L, Romby P, Rees B, Schulze-Briese C, Springer M, Ehresmann C, Ehresmann B, Moras D, Yusupova G, Yusupov M, Science. 2005 Apr 1;308(5718):120-3. PMID:15802605

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