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| {{STRUCTURE_1ynm| PDB=1ynm | SCENE= }} | | {{STRUCTURE_1ynm| PDB=1ynm | SCENE= }} |
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| '''Crystal structure of restriction endonuclease HinP1I'''
| | ===Crystal structure of restriction endonuclease HinP1I=== |
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| ==Overview==
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| HinP1I, a type II restriction endonuclease, recognizes and cleaves a palindromic tetranucleotide sequence (G/CGC) in double-stranded DNA, producing 2 nt 5' overhanging ends. Here, we report the structure of HinP1I crystallized as one protein monomer in the crystallographic asymmetric unit. HinP1I displays an elongated shape, with a conserved catalytic core domain containing an active-site motif of SDX18QXK and a putative DNA-binding domain. Without significant sequence homology, HinP1I displays striking structural similarity to MspI, an endonuclease that cleaves a similar palindromic DNA sequence (C/CGG) and binds to that sequence crystallographically as a monomer. Almost all the structural elements of MspI can be matched in HinP1I, including both the DNA recognition and catalytic elements. Examining the protein-protein interactions in the crystal lattice, HinP1I could be dimerized through two helices located on the opposite side of the protein to the active site, generating a molecule with two active sites and two DNA-binding surfaces opposite one another on the outer surfaces of the dimer. A possible functional link between this unusual dimerization mode and the tetrameric restriction enzymes is discussed.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15805123}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15805123 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15805123}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Dimerizaton]] | | [[Category: Dimerizaton]] |
| [[Category: Restriction endonuclease]] | | [[Category: Restriction endonuclease]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:33:00 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:54:44 2008'' |
