1ypv: Difference between revisions

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[[Image:1ypv.gif|left|200px]]
{{Seed}}
[[Image:1ypv.png|left|200px]]


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{{STRUCTURE_1ypv|  PDB=1ypv  |  SCENE=  }}  
{{STRUCTURE_1ypv|  PDB=1ypv  |  SCENE=  }}  


'''Structure of human thymidylate synthase at low salt conditions'''
===Structure of human thymidylate synthase at low salt conditions===




==Overview==
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Human thymidylate synthase, a target in cancer chemotherapy, was crystallized from PEG 3350 with 30 mM ammonium sulfate (AS) in the crystallization medium. The crystals are isomorphous with the high-salt crystals ( approximately 2.0 M AS) and the structure has been solved and refined (R = 22.6%, R(free) = 24.3%) at 1.8 A resolution. The high- and low-AS-concentration structures are quite similar, with loop 181-197 is in the inactive conformation. Also, residues 95-106 and 129-135 (eukaryotic inserts region) show high mobility as assessed by poor electron density and high values of crystallographic temperature factors (residues 1-25 and 108-129 are disordered in both structures). The high mobility of this region may reflect the situation at physiological ionic strength. Of the four sulfate ions observed bound at 2.0 M AS, only two are present at 30 mM AS. The inactive conformation appears to be stabilized by the side chain of Val3 or a leucine residue from the disordered regions. The low-salt conditions of these crystals should be much more suitable for the study of thymidylate synthase inhibitors, especially those that utilize sulfate-binding sites to stabilize the inactive conformation of loop 181-197.
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{{ABSTRACT_PUBMED_15858273}}


==About this Structure==
==About this Structure==
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[[Category: Methyltransferase]]
[[Category: Methyltransferase]]
[[Category: Thymidylate synthase]]
[[Category: Thymidylate synthase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 16:37:45 2008''
 
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Revision as of 02:12, 28 July 2008

File:1ypv.png

Template:STRUCTURE 1ypv

Structure of human thymidylate synthase at low salt conditions

Template:ABSTRACT PUBMED 15858273

About this Structure

1YPV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of human thymidylate synthase under low-salt conditions., Lovelace LL, Minor W, Lebioda L, Acta Crystallogr D Biol Crystallogr. 2005 May;61(Pt 5):622-7. Epub 2005, Apr 20. PMID:15858273

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