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[[Image:1yzw.gif|left|200px]]
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{{STRUCTURE_1yzw|  PDB=1yzw  |  SCENE=  }}  
{{STRUCTURE_1yzw|  PDB=1yzw  |  SCENE=  }}  


'''The 2.1A Crystal Structure of the Far-red Fluorescent Protein HcRed: Inherent Conformational Flexibility of the Chromophore'''
===The 2.1A Crystal Structure of the Far-red Fluorescent Protein HcRed: Inherent Conformational Flexibility of the Chromophore===




==Overview==
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We have determined the crystal structure of HcRed, a far-red fluorescent protein isolated from Heteractis crispa, to 2.1A resolution. HcRed was observed to form a dimer, in contrast to the monomeric form of green fluorescent protein (GFP) or the tetrameric forms of the GFP-like proteins (eqFP611, Rtms5 and DsRed). Unlike the well-defined chromophore conformation observed in GFP and the GFP-like proteins, the HcRed chromophore was observed to be considerably mobile. Within the HcRed structure, the cyclic tripeptide chromophore, Glu(64)-Tyr(65)-Gly(66), was observed to adopt both a cis coplanar and a trans non-coplanar conformation. As a result of these two conformations, the hydroxyphenyl moiety of the chromophore makes distinct interactions within the interior of the beta-can. These data together with a quantum chemical model of the chromophore, suggest the cis coplanar conformation to be consistent with the fluorescent properties of HcRed, and the trans non-coplanar conformation to be consistent with non-fluorescent properties of hcCP, the chromoprotein parent of HcRed. Moreover, within the GFP-like family, it appears that where conformational freedom is permissible then flexibility in the chromophore conformation is possible.
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{{ABSTRACT_PUBMED_15876379}}


==About this Structure==
==About this Structure==
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[[Category: Wilmann, P G.]]
[[Category: Wilmann, P G.]]
[[Category: Luminescent protein]]
[[Category: Luminescent protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:00:56 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:50:02 2008''

Revision as of 03:50, 29 July 2008

File:1yzw.png

Template:STRUCTURE 1yzw

The 2.1A Crystal Structure of the Far-red Fluorescent Protein HcRed: Inherent Conformational Flexibility of the Chromophore

Template:ABSTRACT PUBMED 15876379

About this Structure

1YZW is a Single protein structure of sequence from Heteractis crispa. Full crystallographic information is available from OCA.

Reference

The 2.1A crystal structure of the far-red fluorescent protein HcRed: inherent conformational flexibility of the chromophore., Wilmann PG, Petersen J, Pettikiriarachchi A, Buckle AM, Smith SC, Olsen S, Perugini MA, Devenish RJ, Prescott M, Rossjohn J, J Mol Biol. 2005 May 27;349(1):223-37. Epub 2005 Mar 22. PMID:15876379

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