1za7: Difference between revisions

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[[Image:1za7.gif|left|200px]]
{{Seed}}
[[Image:1za7.png|left|200px]]


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{{STRUCTURE_1za7|  PDB=1za7  |  SCENE=  }}  
{{STRUCTURE_1za7|  PDB=1za7  |  SCENE=  }}  


'''The crystal structure of salt stable cowpea cholorotic mottle virus at 2.7 angstroms resolution.'''
===The crystal structure of salt stable cowpea cholorotic mottle virus at 2.7 angstroms resolution.===




==Overview==
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Structural transitions in viral capsids play a critical role in the virus life cycle, including assembly, disassembly, and release of the packaged nucleic acid. Cowpea chlorotic mottle virus (CCMV) undergoes a well-studied reversible structural expansion in vitro in which the capsid expands by 10%. The swollen form of the particle can be completely disassembled by increasing the salt concentration to 1 M. Remarkably, a single-residue mutant of the CCMV N-terminal arm, K42R, is not susceptible to dissociation in high salt (salt-stable CCMV [SS-CCMV]) and retains 70% of wild-type infectivity. We present the combined structural and biophysical basis for the chemical stability and viability of the SS-CCMV particles. A 2.7-A resolution crystal structure of the SS-CCMV capsid shows an addition of 660 new intersubunit interactions per particle at the center of the 20 hexameric capsomeres, which are a direct result of the K42R mutation. Protease-based mapping experiments of intact particles demonstrate that both the swollen and closed forms of the wild-type and SS-CCMV particles have highly dynamic N-terminal regions, yet the SS-CCMV particles are more resistant to degradation. Thus, the increase in SS-CCMV particle stability is a result of concentrated tethering of subunits at a local symmetry interface (i.e., quasi-sixfold axes) that does not interfere with the function of other key symmetry interfaces (i.e., fivefold, twofold, quasi-threefold axes). The result is a particle that is still dynamic but insensitive to high salt due to a new series of bonds that are resistant to high ionic strength and preserve the overall particle structure.
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{{ABSTRACT_PUBMED_16537626}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Enhanced local symmetry interactions globally stabilize a mutant virus capsid that maintains infectivity and capsid dynamics., Speir JA, Bothner B, Qu C, Willits DA, Young MJ, Johnson JE, J Virol. 2006 Apr;80(7):3582-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16537626 16537626]
Enhanced local symmetry interactions globally stabilize a mutant virus capsid that maintains infectivity and capsid dynamics., Speir JA, Bothner B, Qu C, Willits DA, Young MJ, Johnson JE, J Virol. 2006 Apr;80(7):3582-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16537626 16537626]
Analysis of a salt stable mutant of cowpea chlorotic mottle virus., Fox JM, Zhao X, Speir JA, Young MJ, Virology. 1996 Aug 1;222(1):115-22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8806492 8806492]
Structures of the native and swollen forms of cowpea chlorotic mottle virus determined by X-ray crystallography and cryo-electron microscopy., Speir JA, Munshi S, Wang G, Baker TS, Johnson JE, Structure. 1995 Jan 15;3(1):63-78. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7743132 7743132]
[[Category: Cowpea chlorotic mottle virus]]
[[Category: Cowpea chlorotic mottle virus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Stable mutant]]
[[Category: Stable mutant]]
[[Category: Stablizing mutation]]
[[Category: Stablizing mutation]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:22:45 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:13:46 2008''

Revision as of 21:13, 28 July 2008

File:1za7.png

Template:STRUCTURE 1za7

The crystal structure of salt stable cowpea cholorotic mottle virus at 2.7 angstroms resolution.

Template:ABSTRACT PUBMED 16537626

About this Structure

1ZA7 is a Single protein structure of sequence from Cowpea chlorotic mottle virus. Full crystallographic information is available from OCA.

Reference

Enhanced local symmetry interactions globally stabilize a mutant virus capsid that maintains infectivity and capsid dynamics., Speir JA, Bothner B, Qu C, Willits DA, Young MJ, Johnson JE, J Virol. 2006 Apr;80(7):3582-91. PMID:16537626

Analysis of a salt stable mutant of cowpea chlorotic mottle virus., Fox JM, Zhao X, Speir JA, Young MJ, Virology. 1996 Aug 1;222(1):115-22. PMID:8806492

Structures of the native and swollen forms of cowpea chlorotic mottle virus determined by X-ray crystallography and cryo-electron microscopy., Speir JA, Munshi S, Wang G, Baker TS, Johnson JE, Structure. 1995 Jan 15;3(1):63-78. PMID:7743132

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