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| [[Image:1zdh.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1zdh| PDB=1zdh | SCENE= }} | | {{STRUCTURE_1zdh| PDB=1zdh | SCENE= }} |
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| '''MS2 COAT PROTEIN/RNA COMPLEX'''
| | ===MS2 COAT PROTEIN/RNA COMPLEX=== |
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| ==Overview==
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| Crystal structures of two complexes between recombinant MS2 capsids and RNA operator fragments have been determined at 2.7 A resolution. The coat protein of the RNA bacteriophage MS2 is bifunctional; it forms the icosahedral virus shell to protect the viral nucleic acid and it acts as a translational repressor by binding with high specificity to a unique site on the RNA, a single stem-loop structure, containing the initiation codon of the gene for the viral replicase. In order to determine the structure of these protein-RNA complexes, we have used chemically synthesized variants of the stem-loop fragment and soaked them into crystals of recombinant capsids. The RNA stem-loop, as bound to the protein, forms a crescent-like structure and interacts with the surface of the beta-sheet of a coat protein dimer. It makes protein contacts with seven phosphate groups on the 5' side of the stem-loop, with a pyrimidine base at position -5, which stacks onto a tyrosine, and with two exposed adenine bases, one in the loop and one at a bulge in the stem. Replacement of the wild-type uridine with a cytosine at position -5 increases the affinity of the RNA to the dimer significantly. The complex with RNA stem-loop having cytosine at this position differs from that of the wild-type complex mainly by having one extra intramolecular RNA interaction and one extra water-mediated hydrogen bond.
| | The line below this paragraph, {{ABSTRACT_PUBMED_9245600}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9245600 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9245600}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rna-binding]] | | [[Category: Rna-binding]] |
| [[Category: Viral protein capsid]] | | [[Category: Viral protein capsid]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:29:40 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:57:36 2008'' |
