1zla: Difference between revisions

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[[Image:1zla.~~gif~~|left|200px]]

[[Image:1zla.png|left|200px]]

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~~'''~~X-ray Structure of a Kaposi's sarcoma herpesvirus LANA peptide bound to the nucleosomal core~~'''~~

===X-ray Structure of a Kaposi's sarcoma herpesvirus LANA peptide bound to the nucleosomal core===

~~==Overview==~~

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~~Kaposi's sarcoma~~-~~associated herpesvirus (KSHV) latency~~-~~associated nuclear antigen (LANA) mediates viral genome attachment~~ to ~~mitotic chromosomes. We find that N-terminal LANA docks onto chromosomes by binding nucleosomes through~~ the ~~folded region of histones H2A-H2B~~. ~~The same LANA residues were required for both H2A-H2B binding and chromosome association~~. ~~Further, LANA did not bind Xenopus sperm chromatin~~, ~~which~~ is ~~deficient in H2A-H2B; chromatin binding was rescued after assembly of nucleosomes containing H2A-H2B. We also describe~~ the 2.9-~~angstrom crystal structure of a nucleosome complexed with the first 23 LANA amino acids. The LANA peptide forms a hairpin that interacts exclusively with an acidic H2A~~-H2B region that is implicated in the formation of higher order chromatin structure. Our findings present a paradigm for how nucleosomes may serve as binding platforms for viral and cellular proteins and reveal a previously unknown mechanism for KSHV latency.

The line below this paragraph, {{ABSTRACT_PUBMED_16469929}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 16469929 is the PubMed ID number.

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==About this Structure==

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[[Category: Nucleosome core particle]]

[[Category: Protein/protein interaction]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 17~~:45:48 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:07:59 2008''

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-[[Image:1zla.gif|left|200px]]
+{{Seed}}
+[[Image:1zla.png|left|200px]]
 <!--
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 {{STRUCTURE_1zla|  PDB=1zla  |  SCENE=  }}
-'''X-ray Structure of a Kaposi's sarcoma herpesvirus LANA peptide bound to the nucleosomal core'''
+===X-ray Structure of a Kaposi's sarcoma herpesvirus LANA peptide bound to the nucleosomal core===
-==Overview==
+<!--
-Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen (LANA) mediates viral genome attachment to mitotic chromosomes. We find that N-terminal LANA docks onto chromosomes by binding nucleosomes through the folded region of histones H2A-H2B. The same LANA residues were required for both H2A-H2B binding and chromosome association. Further, LANA did not bind Xenopus sperm chromatin, which is deficient in H2A-H2B; chromatin binding was rescued after assembly of nucleosomes containing H2A-H2B. We also describe the 2.9-angstrom crystal structure of a nucleosome complexed with the first 23 LANA amino acids. The LANA peptide forms a hairpin that interacts exclusively with an acidic H2A-H2B region that is implicated in the formation of higher order chromatin structure. Our findings present a paradigm for how nucleosomes may serve as binding platforms for viral and cellular proteins and reveal a previously unknown mechanism for KSHV latency.
+The line below this paragraph, {{ABSTRACT_PUBMED_16469929}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 16469929 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_16469929}}
 ==About this Structure==
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 [[Category: Nucleosome core particle]]
 [[Category: Protein/protein interaction]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 17:45:48 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:07:59 2008''