252d: Difference between revisions

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[[Image:252d.gif|left|200px]]
{{Seed}}
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{{STRUCTURE_252d|  PDB=252d  |  SCENE=  }}  
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'''CRYSTAL STRUCTURE OF THE B-DNA DECAMER D(CGCAATTGCG)2; SEQUENCE-DEPENDENT CROSSED HELIX PACKING'''
===CRYSTAL STRUCTURE OF THE B-DNA DECAMER D(CGCAATTGCG)2; SEQUENCE-DEPENDENT CROSSED HELIX PACKING===




==Overview==
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The structure of the B-DNA decamer d(CGCAATTGCG)2 has been determined by X-ray diffraction analysis to a resolution of 2.3 A and an R-factor of 17.7%. The decamer crystallises in the monoclinic space group C2 and packs with a crossed arrangement of helices and a unique crossing contact distinct from all other decamer structures. This is believed to be a direct result of the sequence-dependent minor groove width of the duplex. Crossed helix structures of DNA are valuable starting points for modelling studies of the Holliday junction. Two unique sites are observed at the cross-over junction where strand exchange may occur. A Holliday junction model has been constructed for each case and modelled using molecular mechanics and dynamics techniques. One of these models was found to be fully consistent with the available physical data.
The line below this paragraph, {{ABSTRACT_PUBMED_9223644}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_9223644}}


==About this Structure==
==About this Structure==
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[[Category: B-dna]]
[[Category: B-dna]]
[[Category: Double helix]]
[[Category: Double helix]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:23:19 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:53:05 2008''

Revision as of 16:53, 27 July 2008

File:252d.png

Template:STRUCTURE 252d

CRYSTAL STRUCTURE OF THE B-DNA DECAMER D(CGCAATTGCG)2; SEQUENCE-DEPENDENT CROSSED HELIX PACKING

Template:ABSTRACT PUBMED 9223644

About this Structure

Full crystallographic information is available from OCA.

Reference

Sequence-dependent crossed helix packing in the crystal structure of a B-DNA decamer yields a detailed model for the Holliday junction., Wood AA, Nunn CM, Trent JO, Neidle S, J Mol Biol. 1997 Jun 27;269(5):827-41. PMID:9223644

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