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| {{STRUCTURE_2a1r| PDB=2a1r | SCENE= }} | | {{STRUCTURE_2a1r| PDB=2a1r | SCENE= }} |
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| '''Crystal structure of PARN nuclease domain'''
| | ===Crystal structure of PARN nuclease domain=== |
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| ==Overview==
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| Poly(A)-specific ribonuclease (PARN) is a processive, poly(A)-specific 3' exoribonuclease. The crystal structure of C-terminal truncated human PARN determined in two states (free and RNA-bound forms) reveals that PARNn is folded into two domains, an R3H domain and a nuclease domain similar to those of Pop2p and epsilon186. The high similarity of the active site structures of PARNn and epsilon186 suggests that they may have a similar catalytic mechanism. PARNn forms a tight homodimer, with the R3H domain of one subunit partially enclosing the active site of the other subunit and poly(A) bound in a deep cavity of its nuclease domain in a sequence-nonspecific manner. The R3H domain and, possibly, the cap-binding domain are involved in poly(A) binding but these domains alone do not appear to contribute to poly(A) specificity. Mutations disrupting dimerization abolish both the enzymatic and RNA-binding activities, suggesting that the PARN dimer is a structural and functional unit. The cap-binding domain may act in concert with the R3H domain to amplify the processivity of PARN.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16281054}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16281054 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16281054}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Nuclease domain]] | | [[Category: Nuclease domain]] |
| [[Category: Parn]] | | [[Category: Parn]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:29:41 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:55:45 2008'' |
