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| {{STRUCTURE_2a5y| PDB=2a5y | SCENE= }} | | {{STRUCTURE_2a5y| PDB=2a5y | SCENE= }} |
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| '''Structure of a CED-4/CED-9 complex'''
| | ===Structure of a CED-4/CED-9 complex=== |
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| ==Overview==
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| Interplay among four genes--egl-1, ced-9, ced-4 and ced-3--controls the onset of programmed cell death in the nematode Caenorhabditis elegans. Activation of the cell-killing protease CED-3 requires CED-4. However, CED-4 is constitutively inhibited by CED-9 until its release by EGL-1. Here we report the crystal structure of the CED-4-CED-9 complex at 2.6 A resolution, and a complete reconstitution of the CED-3 activation pathway using homogeneous proteins of CED-4, CED-9 and EGL-1. One molecule of CED-9 binds to an asymmetric dimer of CED-4, but specifically recognizes only one of the two CED-4 molecules. This specific interaction prevents CED-4 from activating CED-3. EGL-1 binding induces pronounced conformational changes in CED-9 that result in the dissociation of the CED-4 dimer from CED-9. The released CED-4 dimer further dimerizes to form a tetramer, which facilitates the autoactivation of CED-3. Together, our studies provide important insights into the regulation of cell death activation in C. elegans.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16208361}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16208361 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16208361}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Ced-4]] | | [[Category: Ced-4]] |
| [[Category: Ced-9]] | | [[Category: Ced-9]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:38:55 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:21:55 2008'' |
