2a7n: Difference between revisions

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[[Image:2a7n.gif|left|200px]]
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[[Image:2a7n.png|left|200px]]


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{{STRUCTURE_2a7n|  PDB=2a7n  |  SCENE=  }}  
{{STRUCTURE_2a7n|  PDB=2a7n  |  SCENE=  }}  


'''Crystal Structure of the G81A mutant of the Active Chimera of (S)-Mandelate Dehydrogenase'''
===Crystal Structure of the G81A mutant of the Active Chimera of (S)-Mandelate Dehydrogenase===


==Overview==
The crystal structures of a soluble mutant of the flavoenzyme mandelate dehydrogenase (MDH) from Pseudomonas putida and of the substrate-reduced enzyme have been analyzed at 1.35-A resolution. The mutant (MDH-GOX2) is a fully active chimeric enzyme in which residues 177-215 of the membrane-bound MDH are replaced by residues 176-195 of glycolate oxidase from spinach. Both structures permit full tracing of the polypeptide backbone chain from residues 4-356, including a 4-residue segment that was disordered in an earlier study of the oxidized protein at 2.15 A resolution. The structures of MDH-GOX2 in the oxidized and reduced states are virtually identical with only a slight increase in the bending angle of the flavin ring upon reduction. The only other structural changes within the protein interior are a 10 degrees rotation of an active site tyrosine side chain, the loss of an active site water, and a significant movement of six other water molecules in the active site by 0.45 to 0.78 A. Consistent with solution studies, there is no apparent binding of either the substrate, mandelate, or the oxidation product, benzoylformate, to the reduced enzyme. The observed structural changes upon enzyme reduction have been interpreted as a rearrangement of the hydrogen bonding pattern within the active site that results from binding of a proton to the N-5 position of the anionic hydroquinone form of the reduced flavin prosthetic group. Implications for the low oxidase activity of the reduced enzyme are also discussed.


==About this Structure==
==About this Structure==
2A7N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7N OCA].  
2A7N is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7N OCA].  


==Reference==
==Reference==
High resolution structures of an oxidized and reduced flavoprotein. The water switch in a soluble form of (S)-mandelate dehydrogenase., Sukumar N, Dewanti AR, Mitra B, Mathews FS, J Biol Chem. 2004 Jan 30;279(5):3749-57. Epub 2003 Nov 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14604988 14604988]
<ref group="xtra">PMID:14604988</ref><ref group="xtra">PMID:11502180</ref><references group="xtra"/>
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
[[Category: Single protein]]
[[Category: Mathews, F S.]]
[[Category: Mathews, F S.]]
[[Category: Mitra, B.]]
[[Category: Mitra, B.]]
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[[Category: Xu, Y.]]
[[Category: Xu, Y.]]
[[Category: Tim barrel]]
[[Category: Tim barrel]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:42:38 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 15:01:34 2009''

Revision as of 13:01, 16 February 2009

File:2a7n.png

Template:STRUCTURE 2a7n

Crystal Structure of the G81A mutant of the Active Chimera of (S)-Mandelate Dehydrogenase

About this Structure

2A7N is a 1 chain structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

  1. Sukumar N, Dewanti AR, Mitra B, Mathews FS. High resolution structures of an oxidized and reduced flavoprotein. The water switch in a soluble form of (S)-mandelate dehydrogenase. J Biol Chem. 2004 Jan 30;279(5):3749-57. Epub 2003 Nov 6. PMID:14604988 doi:https://dx.doi.org/10.1074/jbc.M310049200
  2. Sukumar N, Xu Y, Gatti DL, Mitra B, Mathews FS. Structure of an active soluble mutant of the membrane-associated (S)-mandelate dehydrogenase. Biochemistry. 2001 Aug 21;40(33):9870-8. PMID:11502180

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