2a8p: Difference between revisions

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[[Image:2a8p.~~gif~~|left|200px]]

[[Image:2a8p.png|left|200px]]

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~~'''~~2.7 Angstrom Crystal Structure of the Complex Between the Nuclear SnoRNA Decapping Nudix Hydrolase X29 and Manganese~~'''~~

===2.7 Angstrom Crystal Structure of the Complex Between the Nuclear SnoRNA Decapping Nudix Hydrolase X29 and Manganese===

~~==Overview==~~

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~~X29~~, ~~a 25 kDa Nudix hydrolase from Xenopus laevis that cleaves 5' caps from U8 snoRNA~~, ~~crystallizes as a homodimeric apoenzyme. Manganese binds crystals of apo-X29~~ to ~~form holo-X29 only in~~ the ~~presence of nucleot~~(~~s)ide~~. ~~Structural changes in X29 on nucleo-t(s~~)~~ide-assisted Mn(+2) uptake account for~~ the ~~observed cooperativity of metal binding~~. ~~Structures of X29 with GTP or m7GpppA show a different mode of ligand binding from that of other cap binding proteins and suggest a possible three~~- ~~or four~~-metal Nudix reaction mechanism. The X29 dimer has no known RNA binding motif, but its striking surface dipolarity and unique structural features create a plausible RNA binding channel on the positive face of the protein. Because U8 snoRNP is essential for accumulation of mature 5.8S and 28S rRNA in vertebrate ribosome biogenesis, and cap structures are required for U8 stability in vivo, X29 could profoundly influence this fundamental cellular pathway.

The line below this paragraph, {{ABSTRACT_PUBMED_16472752}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 16472752 is the PubMed ID number.

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==About this Structure==

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[[Category: Wright, H T.]]

[[Category: Modified nudix hydrolase fold]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 18~~:45:09 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:22:24 2008''

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-[[Image:2a8p.gif|left|200px]]
+{{Seed}}
+[[Image:2a8p.png|left|200px]]
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 {{STRUCTURE_2a8p|  PDB=2a8p  |  SCENE=  }}
-'''2.7 Angstrom Crystal Structure of the Complex Between the Nuclear SnoRNA Decapping Nudix Hydrolase X29 and Manganese'''
+===2.7 Angstrom Crystal Structure of the Complex Between the Nuclear SnoRNA Decapping Nudix Hydrolase X29 and Manganese===
-==Overview==
+<!--
-X29, a 25 kDa Nudix hydrolase from Xenopus laevis that cleaves 5' caps from U8 snoRNA, crystallizes as a homodimeric apoenzyme. Manganese binds crystals of apo-X29 to form holo-X29 only in the presence of nucleot(s)ide. Structural changes in X29 on nucleo-t(s)ide-assisted Mn(+2) uptake account for the observed cooperativity of metal binding. Structures of X29 with GTP or m7GpppA show a different mode of ligand binding from that of other cap binding proteins and suggest a possible three- or four-metal Nudix reaction mechanism. The X29 dimer has no known RNA binding motif, but its striking surface dipolarity and unique structural features create a plausible RNA binding channel on the positive face of the protein. Because U8 snoRNP is essential for accumulation of mature 5.8S and 28S rRNA in vertebrate ribosome biogenesis, and cap structures are required for U8 stability in vivo, X29 could profoundly influence this fundamental cellular pathway.
+The line below this paragraph, {{ABSTRACT_PUBMED_16472752}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 16472752 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_16472752}}
 ==About this Structure==
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 [[Category: Wright, H T.]]
 [[Category: Modified nudix hydrolase fold]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:45:09 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:22:24 2008''