2af5: Difference between revisions

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[[Image:2af5.gif|left|200px]]
{{Seed}}
[[Image:2af5.png|left|200px]]


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{{STRUCTURE_2af5|  PDB=2af5  |  SCENE=  }}  
{{STRUCTURE_2af5|  PDB=2af5  |  SCENE=  }}  


'''2.5A X-ray Structure of Engineered OspA protein'''
===2.5A X-ray Structure of Engineered OspA protein===




==Overview==
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Although the beta-rich self-assemblies are a major structural class for polypeptides and the focus of intense research, little is known about their atomic structures and dynamics due to their insoluble and noncrystalline nature. We developed a protein engineering strategy that captures a self-assembly segment in a water-soluble molecule. A predefined number of self-assembling peptide units are linked, and the beta-sheet ends are capped to prevent aggregation, which yields a mono-dispersed soluble protein. We tested this strategy by using Borrelia outer surface protein (OspA) whose single-layer beta-sheet located between two globular domains consists of two beta-hairpin units and thus can be considered as a prototype of self-assembly. We constructed self-assembly mimics of different sizes and determined their atomic structures using x-ray crystallography and NMR spectroscopy. Highly regular beta-sheet geometries were maintained in these structures, and peptide units had a nearly identical conformation, supporting the concept that a peptide in the regular beta-geometry is primed for self-assembly. However, we found small but significant differences in the relative orientation between adjacent peptide units in terms of beta-sheet twist and bend, suggesting their inherent flexibility. Modeling shows how this conformational diversity, when propagated over a large number of peptide units, can lead to a substantial degree of nanoscale polymorphism of self-assemblies.
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{{ABSTRACT_PUBMED_17093048}}


==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Atomic structures of peptide self-assembly mimics., Makabe K, McElheny D, Tereshko V, Hilyard A, Gawlak G, Yan S, Koide A, Koide S, Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17753-8. Epub 2006 Nov 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17093048 17093048]
Atomic structures of peptide self-assembly mimics., Makabe K, McElheny D, Tereshko V, Hilyard A, Gawlak G, Yan S, Koide A, Koide S, Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17753-8. Epub 2006 Nov 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17093048 17093048]
Design of single-layer beta-sheets without a hydrophobic core., Koide S, Huang X, Link K, Koide A, Bu Z, Engelman DM, Nature. 2000 Jan 27;403(6768):456-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10667801 10667801]
[[Category: Borrelia burgdorferi]]
[[Category: Borrelia burgdorferi]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Repeat protein]]
[[Category: Repeat protein]]
[[Category: Single-layer beta-sheet]]
[[Category: Single-layer beta-sheet]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:58:17 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:58:45 2008''
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