2afp: Difference between revisions

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[[Image:2afp.jpg|left|200px]]
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[[Image:2afp.png|left|200px]]


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{{STRUCTURE_2afp|  PDB=2afp  |  SCENE=  }}  
{{STRUCTURE_2afp|  PDB=2afp  |  SCENE=  }}  


'''THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY'''
===THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY===




==Overview==
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A recombinant form of the sea raven type II antifreeze protein (SRAFP) has been produced using the Pichia pastoris expression system. The antifreeze activity of recombinant SRAFP is indistinguishable from that of the wild-type protein. The global fold of SRAFP has been determined by two-dimensional 1H homonuclear and three-dimensional 1H- inverted question mark15N inverted question mark heteronuclear NMR spectroscopy using 785 NOE distance restraints and 47 angular restraints. The molecule folds into one globular domain that consists of two helices and nine beta-strands in two beta-sheets. The structure confirms the proposed existence of five disulfide bonds. The global fold of SRAFP is homologous to C-type lectins and pancreatic stone proteins, even though the sequence identity is only approximately 20%.
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{{ABSTRACT_PUBMED_9537986}}


==About this Structure==
==About this Structure==
2AFP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hemitripterus_americanus Hemitripterus americanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFP OCA].  
2AFP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hemitripterus_americanus Hemitripterus americanus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFP OCA].  


==Reference==
==Reference==
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[[Category: Recombinant sea raven protein]]
[[Category: Recombinant sea raven protein]]
[[Category: Solution backbone fold]]
[[Category: Solution backbone fold]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:59:48 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:25:07 2008''

Revision as of 23:25, 27 July 2008

File:2afp.png

Template:STRUCTURE 2afp

THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY

Template:ABSTRACT PUBMED 9537986

About this Structure

2AFP is a Single protein structure of sequence from Hemitripterus americanus. Full experimental information is available from OCA.

Reference

The solution structure of type II antifreeze protein reveals a new member of the lectin family., Gronwald W, Loewen MC, Lix B, Daugulis AJ, Sonnichsen FD, Davies PL, Sykes BD, Biochemistry. 1998 Apr 7;37(14):4712-21. PMID:9537986

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