2ah7: Difference between revisions

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[[Image:2ah7.gif|left|200px]]
{{Seed}}
[[Image:2ah7.png|left|200px]]


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{{STRUCTURE_2ah7|  PDB=2ah7  |  SCENE=  }}  
{{STRUCTURE_2ah7|  PDB=2ah7  |  SCENE=  }}  


'''Crystal structure of nitrophorin 2 aqua complex'''
===Crystal structure of nitrophorin 2 aqua complex===


==Overview==
Nitrophorin 2 (NP2) (also known as prolixin-S) is a salivary protein that transports nitric oxide, binds histamine, and acts as an anticoagulant during blood feeding by the insect Rhodnius prolixus. The 2.0-A crystal structure of NP2 reveals an eight-stranded antiparallel beta-barrel containing a ferric heme coordinated through His(57), similar to the structures of NP1 and NP4. All four Rhodnius nitrophorins transport NO and sequester histamine through heme binding, but only NP2 acts as an anticoagulant. Here, we demonstrate that recombinant NP2, but not recombinant NP1 or NP4, is a potent anticoagulant; recombinant NP3 also displays minor activity. Comparison of the nitrophorin structures suggests that a surface region near the C terminus and the loops between beta strands B-C and E-F is responsible for the anticoagulant activity. NP2 also displays larger NO association rates and smaller dissociation rates than NP1 and NP4, which may result from a more open and more hydrophobic distal pocket, allowing more rapid solvent reorganization on ligand binding. The NP2 protein core differs from NP1 and NP4 in that buried Glu(53), which allows for larger NO release rates when deprotonated, hydrogen bonds to invariant Tyr(81). Surprisingly, this tyrosine lies on the protein surface in NP1 and NP4.


==About this Structure==
==About this Structure==
2AH7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AH7 OCA].  
2AH7 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AH7 OCA].  


==Reference==
==Reference==
The crystal structure of nitrophorin 2. A trifunctional antihemostatic protein from the saliva of Rhodnius prolixus., Andersen JF, Montfort WR, J Biol Chem. 2000 Sep 29;275(39):30496-503. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10884386 10884386]
<ref group="xtra">PMID:10884386</ref><references group="xtra"/>
[[Category: Rhodnius prolixus]]
[[Category: Rhodnius prolixus]]
[[Category: Single protein]]
[[Category: Berry, R E.]]
[[Category: Berry, R E.]]
[[Category: Montfort, W R.]]
[[Category: Montfort, W R.]]
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[[Category: Ferric heme]]
[[Category: Ferric heme]]
[[Category: Lipocalin]]
[[Category: Lipocalin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:02:39 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 05:35:33 2009''

Revision as of 03:35, 17 February 2009

File:2ah7.png

Template:STRUCTURE 2ah7

Crystal structure of nitrophorin 2 aqua complex

About this Structure

2AH7 is a 1 chain structure of sequence from Rhodnius prolixus. Full crystallographic information is available from OCA.

Reference

  1. Andersen JF, Montfort WR. The crystal structure of nitrophorin 2. A trifunctional antihemostatic protein from the saliva of Rhodnius prolixus. J Biol Chem. 2000 Sep 29;275(39):30496-503. PMID:10884386 doi:https://dx.doi.org/10.1074/jbc.M002857200

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