2ahg: Difference between revisions

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[[Image:2ahg.gif|left|200px]]
{{Seed}}
[[Image:2ahg.png|left|200px]]


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{{STRUCTURE_2ahg|  PDB=2ahg  |  SCENE=  }}  
{{STRUCTURE_2ahg|  PDB=2ahg  |  SCENE=  }}  


'''Unsaturated glucuronyl hydrolase mutant D88N with dGlcA-GalNAc'''
===Unsaturated glucuronyl hydrolase mutant D88N with dGlcA-GalNAc===




==Overview==
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Unsaturated glucuronyl hydrolase (UGL), which is a member of glycoside hydrolase family GH-88, is a bacterial enzyme that degrades mammalian glycosaminoglycans and bacterial biofilms. The enzyme, which acts on unsaturated oligosaccharides with an alpha-glycoside bond produced by microbial polysaccharide lyases responsible for bacterial invasion of host cells, was believed to release 4-deoxy-l-threo-5-hexosulose-uronate (unsaturated glucuronic acid, or DeltaGlcA) and saccharide with a new nonreducing terminus by hydrolyzing the glycosidic bond. We detail the crystal structures of wild-type inactive mutant UGL of Bacillus sp. GL1 and its complex with a substrate (unsaturated chondroitin disaccharide), identify active site residues, and postulate a reaction mechanism catalyzed by UGL that triggers the hydration of the vinyl ether group in DeltaGlcA, based on the structural analysis of the enzyme-substrate complex and biochemical analysis. The proposed catalytic mechanism of UGL is a novel case among known glycosidases. Under the proposed mechanism, Asp-149 acts as a general acid and base catalyst to protonate the DeltaGlcA C4 atom and to deprotonate the water molecule. The deprotonated water molecule attacks the DeltaGlcA C5 atom to yield unstable hemiketal; this is followed by spontaneous conversion to an aldehyde (4-deoxy-l-threo-5-hexosulose-uronate) and saccharide through hemiacetal formation and cleavage of the glycosidic bond. UGL is the first clarified alpha(6)/alpha(6)-barrel enzyme using aspartic acid as the general acid/base catalyst.
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{{ABSTRACT_PUBMED_16893885}}


==About this Structure==
==About this Structure==
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[[Category: Complex with unsaturated chondroitin disaccharide]]
[[Category: Complex with unsaturated chondroitin disaccharide]]
[[Category: Glycoside hydrolase family 88]]
[[Category: Glycoside hydrolase family 88]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:03:10 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:23:07 2008''

Revision as of 08:23, 28 July 2008

File:2ahg.png

Template:STRUCTURE 2ahg

Unsaturated glucuronyl hydrolase mutant D88N with dGlcA-GalNAc

Template:ABSTRACT PUBMED 16893885

About this Structure

2AHG is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.

Reference

Crystal structure of unsaturated glucuronyl hydrolase complexed with substrate: molecular insights into its catalytic reaction mechanism., Itoh T, Hashimoto W, Mikami B, Murata K, J Biol Chem. 2006 Oct 6;281(40):29807-16. Epub 2006 Aug 7. PMID:16893885

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