2ahj: Difference between revisions

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[[Image:2ahj.gif|left|200px]]
{{Seed}}
[[Image:2ahj.png|left|200px]]


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{{STRUCTURE_2ahj|  PDB=2ahj  |  SCENE=  }}  
{{STRUCTURE_2ahj|  PDB=2ahj  |  SCENE=  }}  


'''NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE'''
===NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE===




==Overview==
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The iron-containing nitrile hydratase (NHase) is a photoreactive enzyme that is inactivated in the dark because of persistent association with NO and activated by photo-dissociation of NO. The crystal structure at 1.7 A resolution and mass spectrometry revealed the structure of the non-heme iron catalytic center in the nitrosylated state. Two Cys residues coordinated to the iron were post-translationally modified to Cys-sulfenic and -sulfinic acids. Together with another oxygen atom of the Ser ligand, these modifications induced a claw setting of oxygen atoms capturing an NO molecule. This unprecedented structure is likely to enable the photo-regulation of NHase and will provide an excellent model for designing photo-controllable chelate complexes and, ultimately, proteins.
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{{ABSTRACT_PUBMED_9586994}}


==About this Structure==
==About this Structure==
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[[Category: Photoreactive enzyme]]
[[Category: Photoreactive enzyme]]
[[Category: Post-translational modification of cysteine residue]]
[[Category: Post-translational modification of cysteine residue]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:30:37 2008''

Revision as of 05:30, 29 July 2008

File:2ahj.png

Template:STRUCTURE 2ahj

NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE

Template:ABSTRACT PUBMED 9586994

About this Structure

2AHJ is a Protein complex structure of sequences from Rhodococcus erythropolis. Full crystallographic information is available from OCA.

Reference

Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms., Nagashima S, Nakasako M, Dohmae N, Tsujimura M, Takio K, Odaka M, Yohda M, Kamiya N, Endo I, Nat Struct Biol. 1998 May;5(5):347-51. PMID:9586994

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