2dw4: Difference between revisions

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[[Image:2dw4.~~gif~~|left|200px]]<br />

[[Image:2dw4.jpg|left|200px]]<br /><applet load="2dw4" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="2dw4" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="2dw4, resolution 2.30Å" />

'''Crystal structure of human LSD1 at 2.3 A resolution'''<br />

==Overview==

Transcriptional activity and chromatin structure accessibility are, correlated with the methylation of specific histone residues., Lysine-specific demethylase 1 (LSD1) is the first discovered histone, demethylase, which demethylates Lys4 or Lys9 of histone H3, using FAD., Among the known monoamine oxidase inhibitors, tranylcypromine (Parnate), showed the most potent inhibitory effect on LSD1. Recently, the crystal, structure of LSD1 and tranylcypromine was solved at 2.75A, revealing a, five-membered ring fused to the flavin of LSD1. In this study, we refined, the crystal structure of the LSD1-tranylcypromine complex to 2.25A. The, five-membered ring model did not fit completely with the electron density, giving R(work)/R(free) values of 0.226/0.254. On the other hand, the N(5), adduct gave the lowest R(work)/R(free) values of 0.218/0.248, among the, tested models. These results imply that the LSD1-tranylcypromine complex, is not completely composed of the five-membered adduct, but partially, contains an intermediate, such as the N(5) adduct.

==About this Structure==

2DW4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with FAD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2DW4 OCA].

2DW4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DW4 OCA].

==Reference==

Crystal structure of histone demethylase LSD1 and tranylcypromine at 2.25A., Mimasu S, Sengoku T, Fukuzawa S, Umehara T, Yokoyama S, Biochem Biophys Res Commun. 2008 Feb 1;366(1):15-22. Epub 2007 Nov 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18039463 18039463]

[[Category: Homo sapiens]]

[[Category: Single protein]]

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[[Category: structural genomics]]

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@@ Line 1: / Line 1: @@
-[[Image:2dw4.gif|left|200px]]<br />
+[[Image:2dw4.jpg|left|200px]]<br /><applet load="2dw4" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2dw4" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2dw4, resolution 2.30&Aring;" />
 '''Crystal structure of human LSD1 at 2.3 A resolution'''<br />
+==Overview==
+Transcriptional activity and chromatin structure accessibility are, correlated with the methylation of specific histone residues., Lysine-specific demethylase 1 (LSD1) is the first discovered histone, demethylase, which demethylates Lys4 or Lys9 of histone H3, using FAD., Among the known monoamine oxidase inhibitors, tranylcypromine (Parnate), showed the most potent inhibitory effect on LSD1. Recently, the crystal, structure of LSD1 and tranylcypromine was solved at 2.75A, revealing a, five-membered ring fused to the flavin of LSD1. In this study, we refined, the crystal structure of the LSD1-tranylcypromine complex to 2.25A. The, five-membered ring model did not fit completely with the electron density, giving R(work)/R(free) values of 0.226/0.254. On the other hand, the N(5), adduct gave the lowest R(work)/R(free) values of 0.218/0.248, among the, tested models. These results imply that the LSD1-tranylcypromine complex, is not completely composed of the five-membered adduct, but partially, contains an intermediate, such as the N(5) adduct.
 ==About this Structure==
-DW4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with FAD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2DW4 OCA].
+DW4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DW4 OCA].
+==Reference==
+Crystal structure of histone demethylase LSD1 and tranylcypromine at 2.25A., Mimasu S, Sengoku T, Fukuzawa S, Umehara T, Yokoyama S, Biochem Biophys Res Commun. 2008 Feb 1;366(1):15-22. Epub 2007 Nov 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18039463 18039463]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
@@ Line 26: / Line 31: @@
 [[Category: structural genomics]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 21:40:28 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:18:13 2008''