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| {{STRUCTURE_2an7| PDB=2an7 | SCENE= }} | | {{STRUCTURE_2an7| PDB=2an7 | SCENE= }} |
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| '''Solution structure of the bacterial antidote ParD'''
| | ===Solution structure of the bacterial antidote ParD=== |
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| ==Overview==
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| NMR and CD spectroscopy have been used to characterize, both structurally and dynamically, the 82-amino-acid ParD protein of the post-segregational killing module of the broad-host-range plasmid RP4/RK2. ParD occurs as a dimer in solution and exercises two different control functions; an autoregulatory function by binding to its own promoter P(parDE) and a plasmid-stabilizing function by inhibiting ParE toxicity in cells that express ParD and ParE. Analysis of the secondary structure based on the chemical-shift indices, sequential nuclear Overhauser enhancements (NOEs) and (3)J(Halpha-NH) scalar coupling constants showed that the N-terminal domain of ParD consists of a short beta-ribbon followed by three alpha-helices, demonstrating that ParD contains a ribbon-helix-helix fold, a DNA-binding motif found in a family of small prokaryotic repressors. (15)N longitudinal (T(1)) and transverse (T(2)) relaxation measurements and hetero nuclear NOEs showed that ParD is divided into two separate domains, a well-ordered N-terminal domain and a very flexible C-terminal domain. An increase in secondary structure was observed upon addition of trifluoroethanol, suggested to result from the formation of structured stretches in the C-terminal part of the protein. This is the first experimental evidence that the DNA-binding domain of ParD belongs to the ribbon-helix-helix fold family, and this structural motif is proposed to be present in functionally similar antidote proteins.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17656583}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17656583 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17656583}} |
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| ==About this Structure== | | ==About this Structure== |
| 2AN7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AN7 OCA]. | | 2AN7 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AN7 OCA]. |
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| ==Reference== | | ==Reference== |
| The anti-toxin ParD of plasmid RK2 consists of two structurally distinct moieties and belongs to the ribbon-helix-helix family of DNA-binding proteins., Oberer M, Zangger K, Prytulla S, Keller W, Biochem J. 2002 Jan 1;361(Pt 1):41-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11743881 11743881]
| | <ref group="xtra">PMID:17656583</ref><ref group="xtra">PMID:11743881</ref><references group="xtra"/> |
| [[Category: Escherichia coli]] | | [[Category: Escherichia coli]] |
| [[Category: Single protein]]
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| [[Category: Gruber, K.]] | | [[Category: Gruber, K.]] |
| [[Category: Keller, W.]] | | [[Category: Keller, W.]] |
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| [[Category: Zangger, K.]] | | [[Category: Zangger, K.]] |
| [[Category: Bacterial antidote]] | | [[Category: Bacterial antidote]] |
| | [[Category: Dna binding protein]] |
| [[Category: Dna-binding motif]] | | [[Category: Dna-binding motif]] |
| [[Category: Plasmid addiction]] | | [[Category: Plasmid addiction]] |
| [[Category: Ribbon-helix-helix]] | | [[Category: Ribbon-helix-helix]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:14:49 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 25 08:15:52 2009'' |
