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| {{STRUCTURE_2ask| PDB=2ask | SCENE= }} | | {{STRUCTURE_2ask| PDB=2ask | SCENE= }} |
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| '''Structure of human Artemin'''
| | ===Structure of human Artemin=== |
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| ==Overview==
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| Artemin (ART) promotes the growth of developing peripheral neurons by signaling through a multicomponent receptor complex comprised of a transmembrane tyrosine kinase receptor (cRET) and a specific glycosylphosphatidylinositol-linked co-receptor (GFRalpha3). Glial cell line-derived neurotrophic factor (GDNF) signals through a similar ternary complex but requires heparan sulfate proteoglycans (HSPGs) for full activity. HSPG has not been demonstrated as a requirement for ART signaling. We crystallized ART in the presence of sulfate and solved its structure by isomorphous replacement. The structure reveals ordered sulfate anions bound to arginine residues in the pre-helix and amino-terminal regions that were organized in a triad arrangement characteristic of heparan sulfate. Three residues in the pre-helix were singly or triply substituted with glutamic acid, and the resulting proteins were shown to have reduced heparin-binding affinity that is partly reflected in their ability to activate cRET. This study suggests that ART binds HSPGs and identifies residues that may be involved in HSPG binding.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16734417}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16734417 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16734417}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Neurotrphoic growth factor]] | | [[Category: Neurotrphoic growth factor]] |
| [[Category: Sulfate]] | | [[Category: Sulfate]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:25:34 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:07:43 2008'' |
