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| {{STRUCTURE_2ax2| PDB=2ax2 | SCENE= }} | | {{STRUCTURE_2ax2| PDB=2ax2 | SCENE= }} |
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| '''Production and X-ray crystallographic analysis of fully deuterated human carbonic anhydrase II'''
| | ===Production and X-ray crystallographic analysis of fully deuterated human carbonic anhydrase II=== |
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| ==Overview==
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| Human carbonic anhydrase II (HCA II) is a zinc metalloenzyme that catalyzes the reversible hydration and dehydration of carbon dioxide and bicarbonate, respectively. The rate-limiting step in catalysis is the intramolecular transfer of a proton between the zinc-bound solvent (H2O/OH-) and the proton-shuttling residue His64. This distance (approximately 7.5 A) is spanned by a well defined active-site solvent network stabilized by amino-acid side chains (Tyr7, Asn62, Asn67, Thr199 and Thr200). Despite the availability of high-resolution (approximately 1.0 A) X-ray crystal structures of HCA II, there is currently no definitive information available on the positions and orientations of the H atoms of the solvent network or active-site amino acids and their ionization states. In preparation for neutron diffraction studies to elucidate this hydrogen-bonding network, perdeuterated HCA II has been expressed, purified, crystallized and its X-ray structure determined to 1.5 A resolution. The refined structure is highly isomorphous with hydrogenated HCA II, especially with regard to the active-site architecture and solvent network. This work demonstrates the suitability of these crystals for neutron macromolecular crystallography.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16511248}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16511248 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16511248}} |
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| ==Disease== | | ==Disease== |
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| [[Category: Silverman, D N.]] | | [[Category: Silverman, D N.]] |
| [[Category: Perdeurated human carbonic anhydrase ii proton transfer]] | | [[Category: Perdeurated human carbonic anhydrase ii proton transfer]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:34:35 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:38:26 2008'' |
