2az1: Difference between revisions

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[[Image:2az1.gif|left|200px]]
{{Seed}}
[[Image:2az1.png|left|200px]]


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{{STRUCTURE_2az1|  PDB=2az1  |  SCENE=  }}  
{{STRUCTURE_2az1|  PDB=2az1  |  SCENE=  }}  


'''Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum'''
===Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum===




==Overview==
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Nucleoside diphosphate kinase from the halophilic archaeon Halobacterium salinarum was crystallized in a free state and a substrate-bound form with CDP. The structures were solved to a resolution of 2.35 and 2.2A, respectively. Crystals with the apo-form were obtained with His6-tagged enzyme, whereas the untagged form was used for co-crystallization with the nucleotide. Crosslinking under different salt and pH conditions revealed a stronger oligomerization tendency for the tagged protein at low and high salt concentrations. The influence of the His6-tag on the halophilic nature of the enzyme is discussed on the basis of the observed structural properties.
The line below this paragraph, {{ABSTRACT_PUBMED_16293253}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_16293253}}


==About this Structure==
==About this Structure==
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[[Category: Halophilic]]
[[Category: Halophilic]]
[[Category: Surface charge]]
[[Category: Surface charge]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:38:51 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:51:46 2008''
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