2azm: Difference between revisions

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[[Image:2azm.gif|left|200px]]
{{Seed}}
[[Image:2azm.png|left|200px]]


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{{STRUCTURE_2azm|  PDB=2azm  |  SCENE=  }}  
{{STRUCTURE_2azm|  PDB=2azm  |  SCENE=  }}  


'''Crystal structure of the MDC1 brct repeat in complex with the histone tail of gamma-H2AX'''
===Crystal structure of the MDC1 brct repeat in complex with the histone tail of gamma-H2AX===




==Overview==
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Histone variant H2AX phosphorylation in response to DNA damage is the major signal for recruitment of DNA-damage-response proteins to regions of damaged chromatin. Loss of H2AX causes radiosensitivity, genome instability, and DNA double-strand-break repair defects, yet the mechanisms underlying these phenotypes remain obscure. Here, we demonstrate that mammalian MDC1/NFBD1 directly binds to phospho-H2AX (gammaH2AX) by specifically interacting with the phosphoepitope at the gammaH2AX carboxyl terminus. Moreover, through a combination of biochemical, cell-biological, and X-ray crystallographic approaches, we reveal the molecular details of the MDC1/NFBD1-gammaH2AX complex. These data provide compelling evidence that the MDC1/NFBD1 BRCT repeat domain is the major mediator of gammaH2AX recognition following DNA damage. We further show that MDC1/NFBD1-gammaH2AX complex formation regulates H2AX phosphorylation and is required for normal radioresistance and efficient accumulation of DNA-damage-response proteins on damaged chromatin. Thus, binding of MDC1/NFBD1 to gammaH2AX plays a central role in the mammalian response to DNA damage.
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{{ABSTRACT_PUBMED_16377563}}


==About this Structure==
==About this Structure==
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[[Category: Dna damage]]
[[Category: Dna damage]]
[[Category: Protein-phosphopeptide complex]]
[[Category: Protein-phosphopeptide complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:14:36 2008''

Revision as of 10:14, 27 July 2008

File:2azm.png

Template:STRUCTURE 2azm

Crystal structure of the MDC1 brct repeat in complex with the histone tail of gamma-H2AX

Template:ABSTRACT PUBMED 16377563

About this Structure

2AZM is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

MDC1 directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks., Stucki M, Clapperton JA, Mohammad D, Yaffe MB, Smerdon SJ, Jackson SP, Cell. 2005 Dec 29;123(7):1213-26. PMID:16377563

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