2azt: Difference between revisions

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[[Image:2azt.gif|left|200px]]
{{Seed}}
[[Image:2azt.png|left|200px]]


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{{STRUCTURE_2azt|  PDB=2azt  |  SCENE=  }}  
{{STRUCTURE_2azt|  PDB=2azt  |  SCENE=  }}  


'''Crystal structure of H176N mutant of human Glycine N-Methyltransferase'''
===Crystal structure of H176N mutant of human Glycine N-Methyltransferase===




==Overview==
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In the presence of moderate (2-4 M) urea concentrations the tetrameric enzyme, glycine N-methyltransferase (GNMT), dissociates into compact monomers. Higher concentrations of urea (7-8 M) promote complete denaturation of the enzyme. We report here that the H176N mutation in this enzyme, found in humans with hypermethioninaemia, significantly decreases stability of the tetramer, although H176 is located far from the intersubunit contact areas. Dissociation of the tetramer to compact monomers and unfolding of compact monomers of the mutant protein were detected by circular dichroism, quenching of fluorescence emission, size-exclusion chromatography, and enzyme activity. The values of apparent free energy of dissociation of tetramer and of unfolding of compact monomers for the H176N mutant (27.7 and 4.2 kcal/mol, respectively) are lower than those of wild-type protein (37.5 and 6.2 kcal/mol). A 2.7 A resolution structure of the mutant protein revealed no significant difference in the conformation of the protein near the mutated residue.
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{{ABSTRACT_PUBMED_17660255}}


==Disease==
==Disease==
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[[Category: Wagner, C.]]
[[Category: Wagner, C.]]
[[Category: Glycine n-methyltransferase]]
[[Category: Glycine n-methyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:50:38 2008''

Revision as of 00:50, 28 July 2008

File:2azt.png

Template:STRUCTURE 2azt

Crystal structure of H176N mutant of human Glycine N-Methyltransferase

Template:ABSTRACT PUBMED 17660255

Disease

Known disease associated with this structure: Glycine N-methyltransferase deficiency OMIM:[606628]

About this Structure

2AZT is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Destabilization of human glycine N-methyltransferase by H176N mutation., Luka Z, Pakhomova S, Luka Y, Newcomer ME, Wagner C, Protein Sci. 2007 Sep;16(9):1957-64. Epub 2007 Jul 27. PMID:17660255

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