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| [[Image:2b0j.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2b0j| PDB=2b0j | SCENE= }} | | {{STRUCTURE_2b0j| PDB=2b0j | SCENE= }} |
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| '''The crystal structure of the apoenzyme of the iron-sulfur-cluster-free hydrogenase (Hmd)'''
| | ===The crystal structure of the apoenzyme of the iron-sulfur-cluster-free hydrogenase (Hmd)=== |
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| ==Overview==
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| The iron-sulphur cluster-free hydrogenase (Hmd, EC 1.12.98.2) from methanogenic archaea is a novel type of hydrogenase that tightly binds an iron-containing cofactor. The iron is coordinated by two CO molecules, one sulphur and a pyridone derivative, which is linked via a phosphodiester bond to a guanosine base. We report here on the crystal structure of the Hmd apoenzyme from Methanocaldococcus jannaschii at 1.75 A and from Methanopyrus kandleri at 2.4 A resolution. Homodimeric Hmd reveals a unique architecture composed of one central and two identical peripheral globular units. The central unit is composed of the intertwined C-terminal segments of both subunits, forming a novel intersubunit fold. The two peripheral units consist of the N-terminal domain of each subunit. The Rossmann fold-like structure of the N-terminal domain contains a mononucleotide-binding site, which could harbour the GMP moiety of the cofactor. Another binding site for the iron-containing cofactor is most probably Cys176, which is located at the bottom of a deep intersubunit cleft and which has been shown to be essential for enzyme activity. Adjacent to the iron of the cofactor modelled as a ligand to Cys176, an extended U-shaped extra electron density, interpreted as a polyethyleneglycol fragment, suggests a binding site for the substrate methenyltetrahydromethanopterin.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16540118}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16540118 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16540118}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Helix bundle]] | | [[Category: Helix bundle]] |
| [[Category: Rossmann fold]] | | [[Category: Rossmann fold]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:42:05 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:05:43 2008'' |
