2b2c: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:
[[Image:2b2c.gif|left|200px]]
{{Seed}}
[[Image:2b2c.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2b2c|  PDB=2b2c  |  SCENE=  }}  
{{STRUCTURE_2b2c|  PDB=2b2c  |  SCENE=  }}  


'''Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase'''
===Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase===




==Overview==
<!--
The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned from the model nematode Caenorhabditis elegans. Biochemical characterisation of the recombinantly expressed protein revealed a high degree of similarity to other eukaryotic SPDS with the exception of a low affinity towards the substrate decarboxylated S-adenosylmethionine (Km = 110 microM) and a less pronounced feedback inhibition by the second reaction product 5'-methylthioadenosine (IC50 = 430 microM). The C. elegans protein that carries a nematode-specific insertion of 27 amino acids close to its N-terminus was crystallized, leading to the first X-ray structure of a dimeric eukaryotic SPDS.
The line below this paragraph, {{ABSTRACT_PUBMED_16226262}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 16226262 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_16226262}}


==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Walter, R D.]]
[[Category: Walter, R D.]]
[[Category: Beta-alpha]]
[[Category: Beta-alpha]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:45:48 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:35:36 2008''
Retrieved from "https://proteopedia.org/2b2c"