2b5y: Difference between revisions

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[[Image:2b5y.gif|left|200px]]
{{Seed}}
[[Image:2b5y.png|left|200px]]


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{{STRUCTURE_2b5y|  PDB=2b5y  |  SCENE=  }}  
{{STRUCTURE_2b5y|  PDB=2b5y  |  SCENE=  }}  


'''Solution Structure of a Thioredoxin-like Protein in the Oxidized Form'''
===Solution Structure of a Thioredoxin-like Protein in the Oxidized Form===




==Overview==
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The Bacillus subtilis YkuV responds to environmental oxidative stress and plays an important role for the bacteria to adapt to the environment. Bioinformatic analysis suggests that YkuV is a homolog of membrane-anchored proteins and belongs to the thioredoxin-like protein superfamily containing the typical Cys-Xaa-Xaa-Cys active motif. However, the biological function of this protein remains unknown thus far. In order to elucidate the biological function, we have determined the solution structures of both the oxidized and reduced forms of B. subtilis YkuV by NMR spectroscopy and performed biochemical studies. Our results demonstrated that the reduced YkuV has a low midpoint redox potential, allowing it to reduce a variety of protein substrates. The overall structures of both oxidized and reduced forms are similar, with a typical thioredoxin-like fold. However, significant conformational changes in the Cys-Xaa-Xaa-Cys active motif of the tertiary structures are observed between the two forms. In addition, the backbone dynamics provide further insights in understanding the strong redox potential of the reduced YkuV. Furthermore, we demonstrated that YkuV is able to reduce different protein substrates in vitro. Together, our results clearly established that YkuV may function as a general thiol:disulfide oxidoreductase, which acts as an alternative for thioredoxin or thioredoxin reductase to maintain the reducing environment in the cell cytoplasm.
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{{ABSTRACT_PUBMED_16418167}}


==About this Structure==
==About this Structure==
2B5Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5Y OCA].  
2B5Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5Y OCA].  


==Reference==
==Reference==
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[[Category: Zhang, X.]]
[[Category: Zhang, X.]]
[[Category: Thioredoxin-like]]
[[Category: Thioredoxin-like]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 19:53:56 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:57:48 2008''

Revision as of 12:57, 29 July 2008

File:2b5y.png

Template:STRUCTURE 2b5y

Solution Structure of a Thioredoxin-like Protein in the Oxidized Form

Template:ABSTRACT PUBMED 16418167

About this Structure

2B5Y is a Single protein structure of sequence from Bacillus subtilis. Full experimental information is available from OCA.

Reference

The Bacillus subtilis YkuV is a thiol:disulfide oxidoreductase revealed by its redox structures and activity., Zhang X, Hu Y, Guo X, Lescop E, Li Y, Xia B, Jin C, J Biol Chem. 2006 Mar 24;281(12):8296-304. Epub 2006 Jan 17. PMID:16418167

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