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| {{STRUCTURE_2b75| PDB=2b75 | SCENE= }} | | {{STRUCTURE_2b75| PDB=2b75 | SCENE= }} |
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| '''T4 Lysozyme mutant L99A at 150 MPa'''
| | ===T4 Lysozyme mutant L99A at 150 MPa=== |
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| ==Overview==
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| Formation of a water-expelling nonpolar core is the paradigm of protein folding and stability. Although experiment largely confirms this picture, water buried in "hydrophobic" cavities is required for the function of some proteins. Hydration of the protein core has also been suggested as the mechanism of pressure-induced unfolding. We therefore are led to ask whether even the most nonpolar protein core is truly hydrophobic (i.e., water-repelling). To answer this question we probed the hydration of an approximately 160-A(3), highly hydrophobic cavity created by mutation in T4 lysozyme by using high-pressure crystallography and molecular dynamics simulation. We show that application of modest pressure causes approximately four water molecules to enter the cavity while the protein itself remains essentially unchanged. The highly cooperative filling is primarily due to a small change in bulk water activity, which implies that changing solvent conditions or, equivalently, cavity polarity can dramatically affect interior hydration of proteins and thereby influence both protein activity and folding.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16269539}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16269539 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16269539}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: High pressure]] | | [[Category: High pressure]] |
| [[Category: T4 lysozyme]] | | [[Category: T4 lysozyme]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:56:13 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:16:39 2008'' |
