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| [[Image:2baf.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2baf| PDB=2baf | SCENE= }} | | {{STRUCTURE_2baf| PDB=2baf | SCENE= }} |
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| '''Bovine Fibrinogen alpha-C Domain'''
| | ===Bovine Fibrinogen alpha-C Domain=== |
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| ==Overview==
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| The NMR solution structure of the bovine fibrinogen alphaC-domain fragment, including residues Aalpha374-538, reveals a type-I' beta-hairpin, restricted at the base by a C423-C453 disulfide linkage and a short turn preceding C423. Although both faces of the hairpin are formed mainly by hydrophilic residues, one of them is uncharged while the other has a characteristic pattern of charged residues which are highly conserved among vertebrate species. Chemical shift indexing and relaxation data indicate the presence of a collapsed hydrophobic region next to the hairpin that includes approximately 30 residues with slower concerted motion and higher content of nonpolar residues and, according to a previous study (Tsurupa, G., Tsonev, L., and Medved, L. (2002) Biochemistry 41, 6449-6459), may cooperate with the hairpin to form a compact cooperative unit (domain). Structure and relaxation data show that the region between C423 and C453 is populated by both random coil and beta-structure, suggesting that the cooperative structure in the isolated alphaC-domain is intrinsically unstable. This observation is in agreement with a very low energy of stabilization of the Aalpha374-538 fragment determined in unfolding experiments. The low stability of the alphaC-domain suggests a possible explanation for the previously observed intra- and intermolecular interactions of these domains in fibrinogen and fibrin. | | The line below this paragraph, {{ABSTRACT_PUBMED_16475814}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16475814 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16475814}} |
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| ==About this Structure== | | ==About this Structure== |
| 2BAF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BAF OCA]. | | 2BAF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BAF OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Beta hairpin]] | | [[Category: Beta hairpin]] |
| [[Category: Fibrinogen]] | | [[Category: Fibrinogen]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:02:27 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:44:37 2008'' |
