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| [[Image:2bh5.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2bh5| PDB=2bh5 | SCENE= }} | | {{STRUCTURE_2bh5| PDB=2bh5 | SCENE= }} |
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| '''X-RAY STRUCTURE OF THE M100K VARIANT OF FERRIC CYT C-550 FROM PARACOCCUS VERSUTUS DETERMINED AT 295 K.'''
| | ===X-RAY STRUCTURE OF THE M100K VARIANT OF FERRIC CYT C-550 FROM PARACOCCUS VERSUTUS DETERMINED AT 295 K.=== |
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| ==Overview==
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| The structure of cytochrome c-550 from the nonphotosynthetic bacteria Paraccocus versutus has been solved by X-ray crystallography to 1.90 A resolution, and reveals a high structural homology to other bacterial cytochromes c(2). The effect of replacing the axial heme-iron methionine ligand with a lysine residue on protein structure and unfolding has been assessed using the M100K variant. From X-ray structures at 1.95 and 1.55 A resolution it became clear that the amino group of the lysine side chain coordinates to the heme-iron. Structural differences compared to the wild-type protein are confined to the lysine ligand loop connecting helices four and five. In the heme cavity an additional water molecule is found which participates in an H-bonding interaction with the lysine ligand. Under cryo-conditions extra electron density in the lysine ligand loop is revealed, leading to residues K97 to T101 being modeled with a double main-chain conformation. Upon unfolding, dissociation of the lysine ligand from the heme-iron is shown to be pH dependent, with NMR data consistent with the occurrence of a ligand exchange mechanism similar to that seen for the wild-type protein. | | The line below this paragraph, {{ABSTRACT_PUBMED_15885094}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15885094 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15885094}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Heme]] | | [[Category: Heme]] |
| [[Category: Pyrrolidone carboxylic acid]] | | [[Category: Pyrrolidone carboxylic acid]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:16:49 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:39:03 2008'' |
