2bit: Difference between revisions

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~~'''~~CRYSTAL STRUCTURE OF HUMAN CYCLOPHILIN D AT 1.7 A RESOLUTION~~'''~~

===CRYSTAL STRUCTURE OF HUMAN CYCLOPHILIN D AT 1.7 A RESOLUTION===

~~==Overview==~~

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~~In the pharmaceutical industry~~, ~~knowledge of~~ the ~~three-dimensional structure of a specific target facilitates~~ the ~~drug-discovery process~~. ~~Despite possessing favoured analytical properties such as high purity and monodispersion in light scattering, some proteins are not capable of forming crystals suitable for X-ray analysis~~. ~~Cyclophilin D~~, ~~an isoform of cyclophilin that~~ is ~~expressed in the mitochondria, was selected as a drug target for~~ the ~~treatment of cardiac disorders~~. ~~As the wild-type enzyme defied all attempts at crystallization, protein engineering on the enzyme surface was performed. The K133I mutant gave crystals that diffracted to 1.7 A resolution using in~~-~~house X~~-ray facilities and were suitable for soaking experiments. The crystals were very robust and diffraction was maintained after soaking in 25% DMSO solution: excellent conditions for the rapid analysis of complex structures including crystallographic fragment screening.

The line below this paragraph, {{ABSTRACT_PUBMED_15858260}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 15858260 is the PubMed ID number.

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==About this Structure==

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[[Category: Isomerase]]

[[Category: Mitochondrial protein]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 20~~:21:00 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:36:56 2008''

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-[[Image:2bit.gif|left|200px]]
+{{Seed}}
+[[Image:2bit.png|left|200px]]
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 {{STRUCTURE_2bit|  PDB=2bit  |  SCENE=  }}
-'''CRYSTAL STRUCTURE OF HUMAN CYCLOPHILIN D AT 1.7 A RESOLUTION'''
+===CRYSTAL STRUCTURE OF HUMAN CYCLOPHILIN D AT 1.7 A RESOLUTION===
-==Overview==
+<!--
-In the pharmaceutical industry, knowledge of the three-dimensional structure of a specific target facilitates the drug-discovery process. Despite possessing favoured analytical properties such as high purity and monodispersion in light scattering, some proteins are not capable of forming crystals suitable for X-ray analysis. Cyclophilin D, an isoform of cyclophilin that is expressed in the mitochondria, was selected as a drug target for the treatment of cardiac disorders. As the wild-type enzyme defied all attempts at crystallization, protein engineering on the enzyme surface was performed. The K133I mutant gave crystals that diffracted to 1.7 A resolution using in-house X-ray facilities and were suitable for soaking experiments. The crystals were very robust and diffraction was maintained after soaking in 25% DMSO solution: excellent conditions for the rapid analysis of complex structures including crystallographic fragment screening.
+The line below this paragraph, {{ABSTRACT_PUBMED_15858260}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15858260 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15858260}}
 ==About this Structure==
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 [[Category: Isomerase]]
 [[Category: Mitochondrial protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:21:00 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:36:56 2008''