2bkg: Difference between revisions

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[[Image:2bkg.gif|left|200px]]
{{Seed}}
[[Image:2bkg.png|left|200px]]


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{{STRUCTURE_2bkg|  PDB=2bkg  |  SCENE=  }}  
{{STRUCTURE_2bkg|  PDB=2bkg  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF E3_19 AN DESIGNED ANKYRIN REPEAT PROTEIN'''
===CRYSTAL STRUCTURE OF E3_19 AN DESIGNED ANKYRIN REPEAT PROTEIN===




==Overview==
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Consensus-designed ankyrin repeat (AR) proteins are thermodynamically very stable. The structural analysis of the designed AR protein E3_5 revealed that this stability is due to a regular fold with highly conserved structural motifs and H-bonding networks. However, the designed AR protein E3_19 exhibits a significantly lower stability than E3_5 (9.6 vs. 14.8 kcal/mol), despite 88% sequence identity. To investigate the structural correlations of this stability difference between E3_5 and E3_19, we determined the crystal structure of E3_19 at 1.9 A resolution. E3_19 as well has a regular AR domain fold with the characteristic H-bonding patterns. All structural features of the E3_5 and E3_19 molecules appear to be virtually identical (RMSD(Calpha) approximately 0.7 A). However, clear differences are observed in the surface charge distribution of the two AR proteins. E3_19 features clusters of charged residues and more exposed hydrophobic residues than E3_5. The atomic coordinates of E3_19 have been deposited in the Protein Data Bank. PDB ID: 2BKG.
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{{ABSTRACT_PUBMED_16493627}}


==About this Structure==
==About this Structure==
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[[Category: Designed protein]]
[[Category: Designed protein]]
[[Category: Protein stability]]
[[Category: Protein stability]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:24:42 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:49:05 2008''

Revision as of 05:49, 28 July 2008

File:2bkg.png

Template:STRUCTURE 2bkg

CRYSTAL STRUCTURE OF E3_19 AN DESIGNED ANKYRIN REPEAT PROTEIN

Template:ABSTRACT PUBMED 16493627

About this Structure

2BKG is a Single protein structure. Full crystallographic information is available from OCA.

Reference

Crystal structure of a consensus-designed ankyrin repeat protein: implications for stability., Binz HK, Kohl A, Pluckthun A, Grutter MG, Proteins. 2006 Nov 1;65(2):280-4. PMID:16493627

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